Quantification and confirmation of four Fusarium mycotoxins in corn by gas chromatography-mass spectrometry-selected ion monitoring.
Quantification and confirmation of four Fusarium mycotoxins in corn by gas chromatography-mass spectrometry-selected ion monitoring.
复制标题
通过气相色谱-质谱-选择离子监测对玉米中四种镰刀菌毒素进行定量和确认。
DOI：
10.1016/s0021-9673(00)96257-5
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发表时间：
1984
影响因子：
4.1
通讯作者：
Joseph D. Rosen
中科院分区：
文献类型：
--
作者：
Robert T. Rosen;Joseph D. Rosen
本文建立了同时测定T-2毒素、HT-2毒素、二乙酰氧基卷柏烯醇和玉米赤霉烯酮的快速方法。用甲醇提取玉米样品（10 g），用己烷脱脂，随后使用反相（C，18）和正相（硅胶）Sep-Pak柱进行净化。通过气相色谱-质谱-选择离子监测肌毒素三甲基甲硅烷基衍生物的三种离子特征，确认同一性。氘代内标的使用使得该方法定量可靠并增加灵敏度。可在ca水平下进行鉴别确认和定量。20-50 ppb★★，取决于霉菌毒素。检测限（未确认同一性）估计为1-20 ppb。46-111 ppb水平的回收率范围为80 - 103%，变异系数范围为1.6 - 14.2%。
A rapid method for the simultaneous determination of T-2 toxin, HT-2 toxin, diacetoxyscirpenol and zearalenone has been developed. Corn samples (10 g) are extracted with methanol, defatted with hexane and subsequently cleaned-up using both reversed-phase (C,18) and normal-phase (silica gel) Sep-Pak cartridges. Confirmation of identity is made by gas chromatography-mass spectrometry-selected ion monitoring of three ions characteristic of the trimethylsilyl derivatives of the myocotoxins. Use of deuterated internal standards makes the method quantitavely reliable and increases sensitivity. Confirmation of identity as well as quantitation can be achieved at levels ofca. 20–50 ppb★★, depending on the mycotoxin. Detection limits (without confirmation of identity) are estimated at 1–20 ppb. Recoveries at the 46–111 ppb level ranged from 80 to 103% with coefficients of variation ranging from 1.6 to 14.2%.