Identification of nucleoli in the early branching protist Giardia duodenalis
Identification of nucleoli in the early branching protist Giardia duodenalis
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DOI：
10.1016/j.ijpara.2008.04.012
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发表时间：
2008-09-01
影响因子：
4
通讯作者：
Ortega-Pierres, Guadalupe
中科院分区：
文献类型：
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作者：
Felipe Jimenez-Garcia, Luis;Zavala, Guadalupe;Ortega-Pierres, Guadalupe
十二指肠贾第鞭毛虫被描述为“无核化”。在这项工作中，我们使用电子显微镜和激光共聚焦显微镜的银染和免疫染色技术分析了几种核仁标记在贾第虫核中的亚细胞分布，以及转基因滋养体中表位标记蛋白的表达。我们用招募的核糖核蛋白复合体、rRNA和表位标记的纤维蛋白以及rRNA-伪尿苷合成酶(CBF5)鉴定了与核仁组织区相对应的前核纤维颗粒结构。重组纤维蛋白和CBF5被靶向这个亚室。这项研究证明了十二指肠胃中核仁的存在，并提供了一个模型来分析真核细胞中核仁组装和维持的最低要求。(C)2008澳大利亚寄生虫学会有限公司出版。保留所有权利。
Giardia duodenalis has been described as 'anucleolated'. In this work we analysed the subcellular distribution of several nucleolar markers in Giardia nuclei using silver and immunostaining techniques for electron and confocal laser microscopy as well as expression of epitope-tagged proteins in transgenic trophozoites. We identified anteronuclear fibrogranular structures corresponding to nucleolar organising regions with recruited ribonucleoprotein complexes, rRNA and epitope-tagged fibrillarin and rRNA-pseudouridine synthase (CBF5). Recombinant fibrillarin and CBF5 were targeted to this subcompartment. This study demonstrates the presence of nucleoli in G. duodenalis and provides a model to analyse minimal requirements for nucleolar assembly and maintenance in eukaryotic cells. (C) 2008 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.