Resistance to fungal penetration in Gramineae.
Resistance to fungal penetration in Gramineae.
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禾本科植物对真菌渗透的抵抗力。
DOI：
10.1094/phyto-70-273
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发表时间：
1980
期刊：
影响因子：
3.2
通讯作者：
C. Vance
中科院分区：
文献类型：
--
作者：
R. T. Sherwood;C. Vance
12种在11个部落接种不相容的叶感染真菌。在779的781个表皮部位，其中Stemphyophylobotryosum [Pleospora herbarum]已开始直接渗透，并置发展在表皮细胞壁和渗透是不成功的。当接种的叶子漂浮在放线菌酮（12 μ g/ml），以抑制叶的反应，有渗透767 771个网站，没有壁增厚。因此，放线菌酮抑制沉积和允许表皮渗透。九种还接种了不相容的新月弯孢菌分离物[Cochliobolus lunatus]。在研究的1828次穿透尝试中，1800次显示了无穿透的贴壁，25次显示了无贴壁的穿透，3次显示了通过薄贴壁的穿透。每100个分生孢子的穿透尝试次数为1000次。lunatus从153到314不等。环己酰亚胺导致1469个穿透而无贴壁，52个贴壁而无穿透。在所有的spp。放线菌酮抑制并置的形成，并允许C.月形。当一些spp。接种玉米小斑病菌T小种后，刺入位点表现为贴壁，但无刺入。环己酰亚胺具有相同的效果。附着体的形态和颜色在禾本科植物中差异很大。但在接种3种真菌中的任一种的种内是均匀的。结果表明，禾本科植物具有与表皮相关的组成型和诱导型抗性机制。第一种机制限制渗透频率，对放线菌酮不高度敏感。第二种与并置细胞壁的形成有关，并且是敏感的。附着壁形成的证据是一个一般的机制，在禾本科抗真菌渗透进行了讨论。
Twelve spp. in 11 tribes were inoculated with incompatible leaf-infecting fungi. In 779 of the 781 epidermal sites where Stemphylium botryosum [Pleospora herbarum] had initiated direct penetration, appositions developed in the epidermal cell walls and penetration was unsuccessful. When inoculated leaves were floated on cycloheximide (12 mu g/ml), to inhibit leaf responses, there was penetration in 767 of 771 sites and no wall thickening. Thus cycloheximide inhibited appositions and permitted epidermal penetration. Nine spp. were also inoculated with an incompatible isolate of Curvularia lunata [Cochliobolus lunatus]. Of 1828 penetration attempts studied, 1800 showed appositions without penetrations, 25 showed penetrations without appositions, and 3 showed penetrations through thin appositions. The number of penetration attempts/100 conidia of C. lunatus varied from 153 to 314. Cycloheximide resulted in 1469 penetrations without appositions and 52 appositions without penetrations. In all spp. cycloheximide inhibited the formation of appositions and permitted penetration by C. lunatus. When some spp. were inoculated with Helminthosporium maydis [Cochliobolus heterostrophus] race T the sites of attempted penetration showed appositions without penetration. Cycloheximide had the same effect. The morphology and colour of appositions differed widely among grass spp. but were uniform within a sp. inoculated with any of the 3 fungi. The results indicated that grasses have constitutive and inducible resistance mechanisms associated with the epidermis. The first mechanism restricts frequency of penetration and is not highly sensitive to cycloheximide. The second is correlated with appositional cell wall formation and is sensitive. The evidence that appositional wall formation is a general mechanism of resistance to fungal penetration in the Gramineae is discussed.