Isatis tinctoria mediated synthesis of amphotericin B-bound silver nanoparticles with enhanced photoinduced antileishmanial activity: A novel green approach
Isatis tinctoria mediated synthesis of amphotericin B-bound silver nanoparticles with enhanced photoinduced antileishmanial activity: A novel green approach
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DOI：
10.1016/j.jphotobiol.2016.05.003
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发表时间：
2016-08-01
影响因子：
5.4
通讯作者：
Yuan, Qiping
中科院分区：
文献类型：
--
作者：
Ahmad, Aftab;Wei, Yun;Yuan, Qiping
就死亡率和地理分布而言，利什曼病是继疟疾之后最流行的传染病。有限数量的抗利什曼病药物的可用性、对可用药物的新出现的耐药性以及治疗的高成本使利什曼病的治疗复杂化。为了克服这些问题，需要对具有增强的抗利什曼潜力和低治疗成本的新治疗剂进行关键性研究。在这方面的贡献，我们开发了一个绿色协议，以制备生物银纳米粒子（AgNPs）和阿替霉素B结合的生物银纳米粒子（AmB-AgNPs）。以板蓝根水提物为还原剂和封端剂制备银纳米粒子。两性霉素B成功地吸附在生物银纳米粒子表面。所制备的纳米粒子的特征在于通过各种分析技术。采用紫外-可见光谱检测所制备的纳米颗粒的特征局域表面等离子体共振峰（LSPR）。透射电子显微镜（TEM）和动态光散射（DLS）研究表明，形成的球形银纳米粒子的平均粒径为10-20 nm。通过X射线衍射（XRD）研究证实了所制备的纳米颗粒的立方晶体结构。红外光谱分析表明，植物多酚类化合物主要参与金属还原和封端。在可见光照射下，生物源性银纳米颗粒对热带利什曼原虫表现出显著的活性，其IC 50值为4.2 μ g/mL。这些纳米颗粒的杀利什曼原虫活性通过与阿替霉素B缀合而显著增强（IC 50 = 2.43 μ g/mL）。总之，这项研究的结果表明，吸附的阿替霉素B，抗利什曼原虫药物，生物银纳米粒子，可能是一个安全，更有效和经济的替代现有的抗利什曼原虫的策略。(C)2016爱思唯尔B. V.保留所有权利。
After malaria, Leishmaniasis is the most prevalent infectious disease in terms of fatality and geographical distribution. The availability of a limited number of antileishmanial agents, emerging resistance to the available drugs, and the high cost of treatment complicate the treatment of leishmaniasis. To overcome these issues, critical research for new therapeutic agents with enhanced antileishmanial potential and low treatment cost is needed. In this contribution, we developed a green protocol to prepare biogenic silver nanoparticles (AgNPs) and amphotericin B-bound biogenic silver nanoparticles (AmB-AgNPs). Phytochemicals from the aqueous extract of Isatis tinctoria were used as reducing and capping agents to prepare silver nanoparticles. Amphotericin B was successfully adsorbed on the surface of biogenic silver nanoparticles. The prepared nanoparticles were characterized by various analytical techniques. UV-Visible spectroscopy was employed to detect the characteristic localized surface plasmon resonance peaks (LSPR) for the prepared nanoparticles. Transmission electron microscopy (TEM) and dynamic light scattering (DLS) studies revealed the formation of spherical silver nanoparticles with an average particle size of 10-20 nm. The cubic crystalline structure of the prepared nanoparticles was confirmed by X-ray diffraction (XRD) study. FTIR spectroscopic analysis revealed that plant polyphenolic compounds are mainly involved in metal reduction and capping. Under visible light irradiation, biogenic silver nanoparticles exhibited significant activity against Leishmania tropica with an IC50 value of 4.2 mu g/mL The leishmanicidal activity of these nanoparticles was considerably enhanced by conjugation with amphotericin B (IC50 = 2.43 mu g/mL). In conclusion, the findings of this study reveal that adsorption of amphotericin B, an antileishmanial drug, to biogenic silver nanoparticles, could be a safe, more effective and economic alternative to the available antileishmanial strategies. (C) 2016 Elsevier B.V. All rights reserved.