Crosslinked hyaluronan hydrogels containing mitomycin C reduce postoperative abdominal adhesions
Crosslinked hyaluronan hydrogels containing mitomycin C reduce postoperative abdominal adhesions
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DOI：
10.1016/j.fertnstert.2004.09.038
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发表时间：
2005-04-01
影响因子：
6.7
通讯作者：
Prestwich, GD
中科院分区：
文献类型：
--
作者：
Liu, YC;Li, H;Prestwich, GD
目的：为了评估含有共价结合丝裂霉素C（MMC）的交联透明质酸（HA）水凝胶在大鼠子宫角模型中减少术后粘连的功效，设计：研究两个独立参数：[1]预成型交联水凝胶膜中MMC的量和[2]原位可交联溶液腹膜内注射的功效。大学动物研究机构，动物：雌性Wistar大鼠，干预：在雌性大鼠的子宫内侧壁肌肉组织的接触表面上造成损伤（3 × 10 mm）。使用了两种治疗方案。首先，将含有不同MMC负载（0、0.5%和2%）的无菌交联HA薄膜应用于两个损伤的子宫角;对照动物未接受薄膜。在第二个方案中，将含有不同MMC负载量（0.31%、0.625%和1.25%）的MMC负载交联HA凝胶涂布在子宫角损伤部位（1 mL）;然后，在腹部闭合后将另外4 mL相同制剂注射到腹膜腔中。对照动物仅注射5 mL缓冲液。主要结果测量：子宫角与周围组织和器官之间的术后粘连程度。结果：负载丝裂霉素C的交联HA薄膜和原位交联凝胶在减少术后粘连形成方面比缓冲液对照或不含MMC的交联HA薄膜更有效。结论：负载丝裂霉素C的交联HA膜和凝胶减少了术后腹膜内粘连的形成。（Fertil Steril（（R））2005;83（Suppl 1）：1275-83. (c)2005年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To evaluate the efficacy of crosslinked hyaluronan (HA) hydrogels that contained covalently-bound mitomycin C (MMC) in reducing postoperative adhesions in a rat uterine horn model.Design: Two independent parameters were investigated: [1] the quantity of MMC in preformed crosslinked hydrogel films and [2] the efficacy of intraperitoneal injection of in situ crosslinkable solutions.Setting: University animal research facility.Animal(s) Female Wistar rats.Intervention(s): Injuries (3 x 10 mm) were made to contacting serosal surfaces of the medial uterine wall musculature in female rats. Two treatment protocols were used. In the first, sterile crosslinked HA films that contained different MMC loadings (0, 0.5%, and 2%) were applied to two injured uterine horns; control animals received no films. In the second protocol, MMC-loaded crosslinked HA gels that contained different MMC loadings (0.31%, 0.625%, and 1.25%) were spread on the site of uterine horn injury (1 mL); then, an additional 4 mL of the same formulation was injected into the peritoneal cavity after abdominal closure. Control animals were injected with 5 mL of buffer only.Main Outcome Measure(s): Extent of postoperative adhesions between uterine horns and with surrounding tissues and organs.Result(s): Mitomycin C-loaded crosslinked HA films and in situ crosslinked gels were more effective in reducing postoperative adhesion formation than were buffer controls or crosslinked HA films without MMC.Conclusion(s): Mitomycin C-loaded crosslinked HA films and gels reduced formation of postoperative intraperitoneal adhesions. (Fertil Steril((R)) 2005;83(Suppl 1): 1275-83. (c) 2005 by American Society for Reproductive Medicine.)