Neurochemical characterization of nervous elements innervating the body wall of earthworms (Lumbricus, Eisenia):: immunohistochemical and pharmacological studies
Neurochemical characterization of nervous elements innervating the body wall of earthworms (Lumbricus, Eisenia):: immunohistochemical and pharmacological studies
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DOI：
10.1007/s00441-005-1134-4
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发表时间：
2005-09-01
影响因子：
3.6
通讯作者：
Elekes, K
中科院分区：
文献类型：
--
作者：
Csoknya, M;Takács, B;Elekes, K
本文叙述了蚯蚓体壁神经成分的分布、化学神经解剖和某些生理药理学特性。上皮细胞间可见孤立的感觉双极细胞。这些双极细胞含有各种组合的血清素、酪氨酸羟化酶、组胺、γ-氨基丁酸（GABA）、双羟色胺十四肽、proctolin或视紫红质。在体壁中，肌下丛仅由神经纤维组成，而上皮下丛和肌层丛也含有孤立的神经细胞。这些细胞显示组胺、GABA或神经肽Y免疫反应性。这三个神经丛的纤维对血清素、组胺、神经肽十四肽、proctolin、GABA和神经肽Y抗体具有反应性。肌丛的FMRFamide免疫反应性纤维来源于中枢神经系统，而含有其他研究分子的轴突来源于外周和中枢结构。高压液相色谱分析揭示了体壁中的血清素、多巴胺和组胺。可以用5-羟色胺和FMRFamide引起体壁肌肉组织的收缩。5-羟色胺诱发的收缩被应用GABA抑制。5-羟色胺直接作用于肌细胞受体，并通过引发神经元释放递质间接作用，而FMRFamide诱导的收缩似乎仅通过肌细胞受体介导。5-羟色胺和GABA受体的药理学特征与脊椎动物5-HT 3和GABA（B）受体类型相似。我们的研究结果表明，无论是感觉和传出系统的环节动物体壁操作的各种神经活性化合物的装置，这表明一个复杂的信号系统在这个器官的调节作用。
The distribution and chemical neuroanatomy of nervous elements and certain pharmacological-physiological characteristics of the innervation of the body wall in earthworms are described. Solitary sensory bipolar cells can be found among the epithelial cells. These bipolar cells contain serotonin, tyrosine hydroxylase, histamine, gamma-amino-butyric acid (GABA), Eisenia tetradecapeptide, proctolin or rhodopsin in various combinations. In the body wall, the plexus submuscularis is composed of nerve fibres only, whereas the plexus subepithelialis and muscularis also contain solitary nerve cells. These cells display histamine, GABA or neuropeptide Y immunoreactivity. The fibres of the three plexuses are reactive to serotonin, histamine, Eisenia tetradecapeptide, proctolin, GABA and neuropeptide Y antibodies. FMRFamide-immunoreactive fibres of the plexus muscularis originate from the central nervous system, whereas axons containing the other studied molecules are derived from both peripheral and central structures. High pressure liquid chromatography assays have revealed serotonin, dopamine and histamine in the body wall. Contractions of the body wall musculature can be elicited with serotonin and FMRFamide. Serotonin-evoked contractions are suppressed by the application of GABA. Serotonin acts both directly on the muscle cell receptors and indirectly through initiating transmitter release from the nervous elements, whereas the FMRFamide-induced contractions seem to be mediated through the muscle cell receptors only. The pharmacological profiles of the serotonin and GABA receptors resemble those of the vertebrate 5-HT3 and GABA(B) receptor types. Our findings indicate that both the sensory and efferent system of the annelid body wall operate by means of a variety of neuroactive compounds, suggesting a complex role of signalling systems in the regulation of this organ.