DARK STRESS-INDUCED SENESCENCE OF DETACHED BARLEY LEAVES .2. ALTERATION IN ABSORPTION CHARACTERISTIC AND PHOTOCHEMICAL ACITVITY OF CHLOROPLASTS ISOLATED FROM SENESCING LEAVES
DARK STRESS-INDUCED SENESCENCE OF DETACHED BARLEY LEAVES .2. ALTERATION IN ABSORPTION CHARACTERISTIC AND PHOTOCHEMICAL ACITVITY OF CHLOROPLASTS ISOLATED FROM SENESCING LEAVES
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DOI：
10.1016/0304-4211(76)90094-8
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发表时间：
1976-01-01
期刊：
影响因子：
--
通讯作者：
MOHANTY, P
中科院分区：
文献类型：
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作者：
BISWAL, UC;MOHANTY, P
暗胁迫诱导的衰老引起衰老大麦叶绿体吸收光谱中红色吸收带的红移。IB 65）离开。红移与二氯酚靛酚（DCPIP）与二苯基卡巴肼（DPC）作为电子供体的光还原速率的快速损失在时间上相吻合;这表明暗衰老引起叶绿体的一些结构改变，影响其功能。膜解体剂，当添加到从新鲜叶片分离的叶绿体，导致蓝移，而不是红移，这表明衰老诱导的红移可能不是由于简单的膜结构的破坏。从新鲜叶片分离的叶绿体的热处理引起的红色吸收带的蓝移，在吸收光谱中的红移已经由叶片衰老诱导的叶绿体中没有观察到。激动素和放线菌酮，但不是放线菌素D或氯霉素，防止由老化引起的红移，这意味着参与一些特定的大分子合成与红移。红移的确切性质尚不清楚。
Dark-stress induced senescence causes a red shift in the red absorption band of the absorption spectrum of chloroplasts isolated from senescing barley (Hordeum vulgare L. IB65) leaves. The red shift coincides in time with the rapid loss in the rates of photoreduction of dichlorophenol indophenol (DCPIP) with diphenylcarbazide (DPC) as electron donor; this indicates that dark senescence brings about some structural alterations of chloroplasts which affect their function. Membrane disorganizing agents, when added to the chloroplasts isolated from fresh leaves, cause a blue rather than red shift suggesting that the red shift induced by senescence may not be due to simple disruption of membrane structure. Blue shift of the red absorption band, caused by heat treatment of chloroplasts isolated from fresh leaves, is not observed in the chloroplasts where the red shift in the absorption spectrum was already induced by leaf senescence. Kinetin and cycloheximide, but not actinomycin D or chloramphenicol, prevent the red shift induced by aging which implies involvement of some specific macromolecular synthesis associated with red shift. The exact nature of red shift is not known.