Imaging blood-brain barrier dysfunction as a biomarker for epileptogenesis
Imaging blood-brain barrier dysfunction as a biomarker for epileptogenesis
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DOI：
10.1093/brain/awx073
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发表时间：
2017-06-01
期刊：
影响因子：
14.5
通讯作者：
Friedman, Alon
中科院分区：
文献类型：
--
作者：
Bar-Klein, Guy;Lublinsky, Svetlana;Friedman, Alon
迫切需要一种能够识别发生损伤后癫痫高风险患者的生物标志物。微血管病理学和相关的血脑屏障功能障碍和神经炎症被证明与损伤后癫痫的发生有关。在这里，我们使用前瞻性纵向磁共振成像来定量跟踪癫痫持续状态大鼠的血脑屏障病理学，使用晚期皮质电图来识别癫痫动物，并使用死后免疫组织化学来确认血脑屏障功能障碍和神经炎症。最后，为了测试所提出的生物标志物的药效学相关性，使用了两种抗癫痫干预措施；异氟烷麻醉和氯沙坦。我们的结果表明，梨状网络中的早期血脑屏障病理学是一个敏感且特异的预测因子（曲线下面积为 0.96，P
A biomarker that will enable the identification of patients at high-risk for developing post-injury epilepsy is critically required. Microvascular pathology and related blood-brain barrier dysfunction and neuroinflammation were shown to be associated with epileptogenesis after injury. Here we used prospective, longitudinal magnetic resonance imaging to quantitatively follow blood-brain barrier pathology in rats following status epilepticus, late electrocorticography to identify epileptic animals and post-mortem immunohistochemistry to confirm blood-brain barrier dysfunction and neuroinflammation. Finally, to test the pharmacodynamic relevance of the proposed biomarker, two anti-epileptogenic interventions were used; isoflurane anaesthesia and losartan. Our results show that early blood-brain barrier pathology in the piriform network is a sensitive and specific predictor (area under the curve of 0.96, P