Calcium phosphate nanoparticle adjuvant
Calcium phosphate nanoparticle adjuvant
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DOI：
10.1128/cdli.7.6.899-903.2000
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发表时间：
2000-11-01
期刊：
影响因子：
--
通讯作者：
Bell, SJD
中科院分区：
文献类型：
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作者：
He, Q;Mitchell, AR;Bell, SJD
通过使用能够选择性刺激免疫调节反应的佐剂，可以增强预防人类传染病的疫苗接种。在小鼠模型中，将由磷酸钙（CAP）组成的新型纳米颗粒佐剂与常用的铝（明矾）佐剂就其诱导对单纯疱疹病毒2型（HSV-2）和EB病毒（EBV）感染的免疫力的能力进行比较。结果表明，CAP作为佐剂比明矾更有效，在给药部位引起很少或没有炎症，诱导高滴度的免疫球蛋白G2 a（IgG 2a）抗体和中和抗体，并促进高百分比的抗HSV-2感染的保护。CAP的其他益处包括（i）不显著的IgE应答，这是相对于注射明矾化合物的重要优势，和（ii）CAP是人体的天然成分的事实。因此，CAP具有良好的耐受性和吸收性。这些研究是用动物模型进行的。凭借这种CAP佐剂的效力和相对不存在副作用，我们相信这种新的CAP制剂具有用作人类佐剂的巨大潜力。
Vaccination to protect against human infectious diseases may be enhanced by using adjuvants that can selectively stimulate immunoregulatory responses. In a murine model, a novel nanoparticulate adjuvant composed of calcium phosphate (CAP) was compared with the commonly used aluminum (alum) adjuvants for its ability to induce immunity to herpes simplex virus type 2 (HSV-2) and Epstein-Barr virus (EBV) infections. Results indicated that CAP was more potent as an adjuvant than alum, elicited little or no inflammation at the site of administration, induced high titers of immunoglobulin G2a (IgG2a) antibody and neutralizing antibody, and facilitated a high percentage of protection against HSV-2 infection. Additional benefits of CAP include (i) an insignificant IgE response, which is an important advantage over injection of alum compounds, and (ii) the fact that CAP is a natural constituent of the human body. Thus, CAP is very well tolerated and absorbed. These studies were performed with animal models. By virtue of the potency of this CAP adjuvant and the relative absence of side effects, we believe that this new CAP formulation has great potential for use as an adjuvant in humans.