Prediction of the coding sequences of unidentified human genes.: XIX.: The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro
Prediction of the coding sequences of unidentified human genes.: XIX.: The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro
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DOI：
10.1093/dnares/7.6.347
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发表时间：
2000-12-31
期刊：
影响因子：
4.1
通讯作者：
Ohara, O
中科院分区：
文献类型：
--
作者：
Nagase, T;Kikuno, R;Ohara, O
作为我们的人类cDNA计划的一个扩展，积累未知基因的编码序列的序列信息，我们在这里提出了100个未知基因的cDNA克隆的完整序列，命名为KIAA1673-KIAA1772，来自三套大小分级cDNA文库来自人成人全脑，海马，和胎儿全脑。插入片段和相应的开放阅读框的平均大小分别为4.9 kb和2.7 kb（对应于895个氨基酸残基）。通过计算机辅助分析推导的氨基酸序列，44个预测的基因产物被分为5个功能类别的蛋白质相关的细胞信号/通信，核酸管理，细胞结构/运动，蛋白质管理和代谢。此外，还研究了基因的表达谱在10个人体组织，8个脑区，脊髓，胎脑和胎肝通过逆转录-偶联聚合酶链反应，其产物通过酶联免疫吸附测定定量。
As an extension of our human cDNA project for accumulating sequence information on the coding sequences of unidentified genes, we here present the entire sequences of 100 cDNA clones of unidentified genes, named KIAA1673-KIAA1772, from three sets of size-fractionated cDNA libraries derived from human adult whole brain, hippocampus, and fetal whole brain. The average sizes of the inserts and corresponding open reading frames of cDNA clones analyzed here were 4.9 kb and 2.7 kb (corresponding to 895 amino acid residues), respectively. By computer-assisted analysis of the deduced amino acid sequences, 44 predicted gene products were classified into five functional categories of proteins relating to cell signaling/communication, nucleic acid management, cell structure/motility, protein management, and metabolism. Furthermore, the expression profiles of the genes were also studied in 10 human tissues, 8 brain regions, spinal cord, fetal brain and fetal liver by reverse-transcription-coupled polymerase chain reaction, the products of which were quantified by enzyme-linked immunosorbent assay.