A RAF-SnRK2 kinase cascade mediates early osmotic stress signaling in higher plants
A RAF-SnRK2 kinase cascade mediates early osmotic stress signaling in higher plants
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RAF-SnRK2 激酶级联介导高等植物早期渗透胁迫信号传导
DOI：
10.1038/s41467-020-14477-9
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发表时间：
2020-01-30
影响因子：
16.6
通讯作者：
Wang, Pengcheng
中科院分区：
文献类型：
--
作者：
Lin, Zhen;Li, Yuan;Wang, Pengcheng
渗透调节对于植物生长发育和对环境变化的响应具有重要意义。SNF 1相关蛋白激酶2s（SnRK 2s）在渗透胁迫下被迅速激活，是渗透胁迫和脱落酸（阿坝）信号通路中的核心成分，但SnRK 2激活和早期渗透胁迫信号通路所需的上游成分仍不清楚。本文报道了Raf-like kinases（RAFs）B2、B3和B4亚家族在拟南芥早期渗透胁迫和阿坝信号转导中的重要作用。B2、B3和B4 RAFs被渗透压应激快速激活，并且是SnRK 2的磷酸化和激活所必需的。RAFs高阶突变体的分析揭示了RAFs在渗透胁迫耐受性和阿坝响应以及生长发育中的关键作用。我们的研究结果揭示了一个激酶级联介导的调节高等植物中。
Osmoregulation is important for plant growth, development and response to environmental changes. SNF1-related protein kinase 2s (SnRK2s) are quickly activated by osmotic stress and are central components in osmotic stress and abscisic acid (ABA) signaling pathways; however, the upstream components required for SnRK2 activation and early osmotic stress signaling are still unknown. Here, we report a critical role for B2, B3 and B4 subfamilies of Raf-like kinases (RAFs) in early osmotic stress as well as ABA signaling inArabidopsis thaliana. B2, B3 and B4 RAFs are quickly activated by osmotic stress and are required for phosphorylation and activation of SnRK2s. Analyses of high-order mutants ofRAFsreveal critical roles of the RAFs in osmotic stress tolerance and ABA responses as well as in growth and development. Our findings uncover a kinase cascade mediating osmoregulation in higher plants.