Purification and further characterization of an anti-murine interferon-gamma monoclonal neutralizing antibody.
Purification and further characterization of an anti-murine interferon-gamma monoclonal neutralizing antibody.
复制标题
抗鼠干扰素-γ单克隆中和抗体的纯化和进一步表征。
DOI：
10.1089/jir.1986.6.489
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Havell,EA
中科院分区：
文献类型：
--
作者：
Havell,EA
R4-6A2 大鼠-小鼠杂交瘤分泌能够中和鼠干扰素-γ (MuIFN-γ) 的大鼠 IgG1 单克隆抗体 (MAb)，在无胸腺裸鼠 (nu/nu) 中以腹水形式生长。腹水的中和滴度比 R4-6A2 培养物上清液的中和滴度高 400 倍。此外，腹水的比活性（MAb中和单位/mg蛋白质）远大于R4-6A2培养上清液的比活性。腹水单克隆抗体通过阴离子交换色谱纯化，所应用的中和活性具有良好的恢复。研究了 R4-6A2 抗 MuIFN-γ MAb 中和 MuIFN-γ 几种不同活性的能力。 MAb 中和了 MuIFN-γ 抑制细胞生长的能力（抗增殖活性）。 MuIFN-γ 使异源物种（大鼠）细胞抵抗病毒复制的能力也被该 MAb 中和。 MuIFN-γ 抗病毒活性的 MAb 中和滴度，在同源（鼠 L929）和异源（大鼠成纤维细胞）细胞上，与每种细胞类型的抗病毒活性滴度成反比。
The R4-6A2 rat-mouse hybridoma, which secretes a rat IgG1monoclonal antibody (MAb) capable of neutralizing murine interferon-γ (MuIFN-γ), was grown as ascites in athymic nude (nu/nu) mice. The neutralizing titers of ascitic fluids were 400 times higher than those of supernatants from R4-6A2 cultures. Moreover, the specific activities (MAb neutralizing units/mg protein) of the ascitic fluids were much greater than those of R4-6A2 culture supernatants. Ascitic fluid MAb was purified by anion-exchange Chromatography, with excellent recovery of applied neutralizing activity. The ability of the R4-6A2 anti-MuIFN-γ MAb to neutralize several different activities of MuIFN-γ was studied. The MAb neutralized the ability of MuIFN-γ to inhibit the growth of cells (antiproliferative activity). The ability of MuIFN-γ to render cells of a heterologous species (rat) resistant to viral replication was also neutralized by this MAb. The MAb-neutralizing titers for MuIFN-γ antiviral activities, on both homologous (murine L929) and heterologous (rat fibroblast) cells, were inversely proportional to the antiviral activity titers on each cell type.