DNA binding-uptake system: a link between cell-to-cell communication and biofilm formation
DNA binding-uptake system: a link between cell-to-cell communication and biofilm formation
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DOI：
10.1128/jb.187.13.4392-4400.2005
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发表时间：
2005-07-01
影响因子：
3.2
通讯作者：
Scheie, AA
中科院分区：
文献类型：
--
作者：
Petersen, FC;Tao, L;Scheie, AA
DNA最近被描述为生物膜中细胞外基质的主要结构成分。在链球菌中，细胞间的能力刺激肽（CSP）信号参与了遗传转化、生物膜形成和自溶的能力。在响应CSP调控的基因中，包括那些参与结合和摄取细胞外DNA的基因。我们在这项研究中表明，一个功能性的DNA结合摄取系统参与了生物膜的形成。变形链球菌的comGB突变体缺乏DNA结合和摄取，但信号传导不受影响，显示生物膜形成减少。在DNA酶1存在下的生长过程中，野生型的生物膜减少到与comGB突变体相似的水平，这表明DNA在野生型生物膜的形成中起重要作用。我们还发现，在合成CSP存在下的生长促进了DNA的显著释放，在野生型和comGB突变体中具有相似的水平。DNA结合摄取系统在生物膜形成中的重要性指出了可能对抗感染的新靶点。
DNA has recently been described as a major structural component of the extracellular matrix in biofilms. In streptococci, the competence-stimulating peptide (CSP) cell-to-cell signal is involved in competence for genetic transformation, biofilm formation, and autolysis. Among the genes regulated in response to the CSP are those involved in binding and uptake of extracellular DNA. We show in this study that a functional DNA binding-uptake system is involved in biofilm formation. A comGB mutant of Streptococcus mutans deficient in DNA binding and uptake, but unaffected in signaling, showed reduced biofilm formation. During growth in the presence of DNase 1, biofilm was reduced in the wild type to levels similar to those found with the comGB mutant, suggesting that DNA plays an important role in the wild-type biofilm formation. We also showed that growth in the presence of synthetic CSP promoted significant release of DNA, with similar levels in the wild type and in the comGB mutant. The importance of the DNA binding-uptake system in biofilm formation points to possible novel targets to fight infections.