Biofunctional Quantum Dots: Controlled Conjugation for Multiplexed Biosensors
Biofunctional Quantum Dots: Controlled Conjugation for Multiplexed Biosensors
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DOI：
10.1021/nn2023123
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发表时间：
2011-07-01
期刊：
影响因子：
17.1
通讯作者：
Hildebrandt, Niko
中科院分区：
文献类型：
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作者：
Hildebrandt, Niko
半导体量子点具有独特的光物理特性，如窄波长带宽的明亮发射和极宽强的吸收。结合其尺寸依赖的颜色可调性，量子点已被提出作为多路光学生物分析的理想候选者超过十年。然而，稳定的、可复制的、具有可控制和功能的多重生物标记的生物相容性量子点的缺乏限制了这些纳米晶体的研究应用。在本期ACS Nano中，Jennings等人展示了商用试剂盒生产的量子点抗体偶联物的多种用途，这些试剂盒可以轻松快速地进行标记。这一观点强调了新型量子点生物偶联方法与最先进的检测方法和技术相结合的潜力，这些方法和技术成功且广泛应用于多路生物传感。
Semiconductor quantum dots possess unique photophysical properties such as bright emission with narrow wavelength bandwidth and extremely broad and strong absorption. In combination with their size-dependent color tunability, quantum dots have been proposed as ideal candidates for multiplexed optical bioanalysis for more than a decade. However, the unavailability of stable, reproducible, biocompatible quantum dots with controlled and functional multiple biolabeling has restricted these nanocrystals to research applications. In this issue of ACS Nano, Jennings et al. demonstrate the versatile use of quantum dot antibody conjugates produced by commercially available kits that allow an easy and fast labeling. This Perspective highlights the potential of novel quantum dot bioconjugation approaches in combination with state-of-the-art detection methods and technologies for successful and widely applicable multiplexed biosensing.