Enhanced 2,3-butanediol production by Klebsiella oxytoca using a two-stage agitation speed control strategy
Enhanced 2,3-butanediol production by Klebsiella oxytoca using a two-stage agitation speed control strategy
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使用两级搅拌速度控制策略提高产酸克雷伯氏菌的 2,3-丁二醇产量。
DOI：
10.1016/j.biortech.2009.02.031
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发表时间：
2009-07-01
影响因子：
11.4
通讯作者：
Li, Shuang
中科院分区：
文献类型：
--
作者：
Ji, Xiao-Jun;Huang, He;Li, Shuang
以产酸克雷伯氏菌（Klebsiella oxytoca）为研究对象，通过改变搅拌速度，采用不同的供氧方式，对产酸克雷伯氏菌分批发酵生产2，3-丁二醇进行了研究。通过对细胞比生长速率（mu）等三个动力学参数的分析，确定了细胞生长的最佳条件。比葡萄糖消耗速率（q（s））和比2，3-丁二醇生成速率（q（p）），提出了一种两级搅拌速度控制策略，旨在实现2，3-丁二醇的高浓度、高收率和高生产率。在前15小时，将搅拌速度控制在300 rpm以获得细胞生长的高mu，随后将搅拌速度控制在200 rpm以维持高q（p）以获得高2.3-丁二醇积累。最后，2，3-丁二醇的最高浓度达到95.5 g l（-1），产率为0.478 g g（-1），生产率为1.71 g l（-1）h（-1），分别比恒转速控制的最佳结果高6.23%、6.22%和22.14%。皇冠版权所有（C）2009由爱思唯尔有限公司出版。保留所有权利。
Batch fermentative production of 2,3-butanediol by Klebsiella oxytoca was investigated using various oxygen supply methods though varying agitation speed. Based on the analysis of three kinetic parameters including specific cell growth rate (mu). specific glucose consumption rate (q(s)) and specific 2,3-butanediol formation rate (q(p)), a two-stage agitation speed control strategy, aimed at achieving high concentration, high yield and high productivity of 2.3-butanediol, was proposed. At the first 15 h, agitation speed was controlled at 300 rpm to obtain high mu for cell growth, subsequently agitation speed was controlled at 200 rpm to maintain high q(p) for high 2.3-butanediol accumulation. Finally, the maximum concentration of 2,3-butanediol reached 95.5 g l(-1) with the yield of 0.478 g g(-1) and the productivity of 1.71 g l(-1) h(-1), which were 6.23%, 6.22% and 22.14% over the best results controlled by constant agitation speeds. Crown Copyright (C) 2009 Published by Elsevier Ltd. All rights reserved.