Migration of lymphocytes on fibronectin-coated surfaces: temporal evolution of migratory parameters
Migration of lymphocytes on fibronectin-coated surfaces: temporal evolution of migratory parameters
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DOI：
10.1016/s0142-9612(99)00154-4
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发表时间：
1999-12-01
期刊：
影响因子：
14
通讯作者：
Zygourakis, K
中科院分区：
文献类型：
--
作者：
Bergman, AJ;Zygourakis, K
淋巴细胞通常通过吸附的外源蛋白质与植入的生物材料相互作用。为了更全面地描述这些相互作用，在进行常见的黏附研究的同时，必须对淋巴细胞在吸附的细胞外基质蛋白质上的迁移进行分析。我们在此报道了Jurkat细胞（一种T淋巴母细胞系）在经组织培养处理和未经处理的、涂有不同浓度纤连蛋白的聚苯乙烯表面上的迁移和黏附行为的比较。细胞移动的平均速度对于在未经处理的聚苯乙烯上迁移的细胞表现出对底物黏附性的双相反应，而对于在经组织培养处理的聚苯乙烯上迁移的细胞则单调下降。我们采用了一种改进的持续随机游走模型方法来确定细胞迁移参数的时间依赖性。当细胞在经组织培养处理的聚苯乙烯表面上迁移时，随机运动系数随时间显著增加，而在使用未经处理的聚苯乙烯板的实验中，该系数则相对保持恒定。最后，我们开发了一个细胞迁移计算机模型来验证我们改进的持续随机游走分析。模拟结果表明，我们的实验数据与随机运动系数随时间增加是一致的。（C）1999年爱思唯尔科学有限公司。保留所有权利。
Lymphocytes typically interact with implanted biomaterials through adsorbed exogenous proteins. To provide a more complete characterization of these interactions, analysis of lymphocyte migration on adsorbed extracellular matrix proteins must accompany the commonly performed adhesion studies. We report here a comparison of the migratory and adhesion behavior of Jurkat cells (a T lymphoblastoid cell line) on tissue culture treated and untreated polystyrene surfaces coated with Various concentrations of fibronectin. The average speed of cell locomotion showed a biphasic response to substrate adhesiveness for cells migrating on untreated polystyrene and a monotonic decrease for cells migrating on tissue culture-treated polystyrene. A modified approach to the persistent random walk model was implemented to determine the time dependence of cell migration parameters. The random motility coefficient showed significant increases with time when cells migrated on tissue culture-treated polystyrene surfaces, while it remained relatively constant for experiments with untreated polystyrene plates. Finally, a cell migration computer model was developed to verify our modified persistent random walk analysis. Simulation results suggest that our experimental data were consistent with temporally increasing random motility coefficients. (C) 1999 Elsevier Science Ltd. All rights reserved.