Guidelines for Experiments Using Antisense Oligonucleotides and Double-Stranded RNAs
Guidelines for Experiments Using Antisense Oligonucleotides and Double-Stranded RNAs
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DOI：
10.1089/nat.2018.0772
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发表时间：
2019-03-23
影响因子：
4
通讯作者：
Corey, David R.
中科院分区：
文献类型：
--
作者：
Gagnon, Keith T.;Corey, David R.
经过几十年的研究和开发，合成核酸开始在临床上取得重大成功。批准的药物增加了该领域的兴趣，许多基础研究集中在合成核酸来控制信使RNA和非编码RNA的活动。不幸的是，实验设计往往不充分，导致对数据的误导解释和缺乏说服力的工作，浪费资源，对该领域的发展几乎没有什么帮助。这篇评论的目的是概述许多研究人员面临的问题，特别是那些对合成寡核苷酸的使用不熟悉的人。我们描述了必要的最低限度的控制实验，以建立一个强有力的案例，真正的影响，可能是由于相互作用在预定的分子目标。准备和判断实验的一套共同标准应有助于更好地解释数据和出版物，这些数据和出版物对将合成核酸用作工具和药物作出了积极贡献。
After decades of research and development, synthetic nucleic acids are beginning to enjoy significant success in the clinic. Approved drugs have increased interest in the field, and many basic research studies have focused on synthetic nucleic acids to control the action of messenger RNA and noncoding RNAs. Unfortunately, experimental designs are often inadequate, resulting in misleading interpretation of data and unconvincing work that wastes resources and does little to advance the field. The goal of this commentary is to outline the problems facing many researchers, especially those new to the use of synthetic oligonucleotides. We describe the minimum control experiments necessary to build a strong case for real effects that are likely due to interactions at the intended molecular target. A common set of standards for preparing and judging experiments should facilitate better interpretation of data and publications that contribute positively to using synthetic nucleic acids as tools and drugs.