ANALYSIS OF MONOCLONAL IMMUNE-RESPONSE TO INFLUENZA-VIRUS .1. PRODUCTION OF MONOCLONAL ANTI-VIRAL ANTIBODIES INVITRO
ANALYSIS OF MONOCLONAL IMMUNE-RESPONSE TO INFLUENZA-VIRUS .1. PRODUCTION OF MONOCLONAL ANTI-VIRAL ANTIBODIES INVITRO
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DOI：
10.1002/eji.1830051013
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发表时间：
1975-01-01
影响因子：
5.4
通讯作者：
KLINMAN, NR
中科院分区：
文献类型：
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作者：
GERHARD, W;BRACIALE, TJ;KLINMAN, NR
接种流感病毒的小鼠的有限数量的脾细胞被转移到致死照射的同基因受者体内。对受体脾的片段培养物进行抗原刺激，观察到转移的脾细胞数与反应片段数之间存在线性关系。单个片段的抗体产物在等电聚焦以及与各种病毒抗原决定簇的反应性方面表现出高度受限的异质性。结论：这种过继转移系统中的极限细胞类型对应于病毒诱导的B细胞，B细胞在抗原刺激下产生抗体产生细胞的克隆。在PR8免疫的BALB/c小鼠中，PR8特异性前体B细胞的出现频率至少为1/4000。大约25%的刺激细胞克隆产生了足够数量的抗体(200毫微克)，使分析密切相关的病毒抗原成为可能。
Limiting numbers of spleen cells from mice primed with influenza virus were transferred into lethally irradiated syngeneic recipients. Upon antigenic stimulation of fragment cultures of the recipient spleens it was observed that a linear relationship existed between the number of transferred spleen cells and the number of responding fragments. The antibody product of individual fragments exhibited a highly restricted heterogeneity in isoelectric focusing as well as in its reactivity against various viral antigenic determinants. It was concluded that the limiting cell type in this adoptive transfer system corresponds to the virus‐primed B cell which, upon antigenic stimulation, gives rise to a clone of antibody‐producing cells. PR8‐specific precursor B cells occurred at a frequency of at least 1/4000 splenic B cells in PR8‐primed BALB/c mice. Approximately 25% of the stimulated cell clones produced sufficient quantities of antibody (200 ng) to render feasible an analysis of closely related viral antigens.