Oxygen-Enriched Photosensitizer Medium with Red Blood Cells to Study Tissue Interaction of Photosensitization Reaction
Oxygen-Enriched Photosensitizer Medium with Red Blood Cells to Study Tissue Interaction of Photosensitization Reaction
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富氧光敏剂培养基与红细胞研究光敏反应的组织相互作用
DOI：
10.1089/pho.2017.4321
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发表时间：
2018
影响因子：
--
通讯作者：
Arai Tsunenori
中科院分区：
文献类型：
--
作者：
Hamada Risa;Ogawa Emiyu;Arai Tsunenori
背景：据报道，在辐照过程中，光敏剂介质的氧分压降低，导致光敏反应对靶细胞的效力降低。目的：本研究的目的是获得具有高溶解氧水平的培养威尔斯孔与感知氧环境和光敏剂漂白的光敏反应studies.Materials和方法：我们使用了一个10 mm宽的光学细胞比色皿与1 mm的光路长度的井。采用红细胞比容水平为0.625%的红细胞（RBC）悬浮液作为光学样品。加入光敏剂他拉泊芬钠至浓度为30 μg/mL。用663-nm二极管激光器以120 mW/cm 2照射光学样品，总辐射暴露量为0-20 J/cm 2，以诱导光敏化反应。在每个照射条件之前和之后测量样品在475-700 nm范围内的吸收光谱。选择可见光谱来区分主要的三种血红蛋白（Hb）类型：氧合血红蛋白、脱氧血红蛋白和高铁血红蛋白。此外，该波长范围被选择用于研究使用Q带吸收峰的光漂白。每个血红蛋白浓度估计使用多元回归分析应用到获得的absorptionspectross.Results：氧饱和度和Q波段的吸收峰之间的关系，从他拉泊芬钠的动力学与辐射exposure.Conclusions：我们的方法揭示了约两倍的氧溶解solution：我们可以感知的氧环境和光敏化反应的进展，同时增加溶解氧通过添加红细胞的细胞培养基和测量它的吸收光谱。
Background:It has been reported that the oxygen pressure of a photosensitizer medium decreases during an irradiation leading to decrease in the efficacy of the photosensitization reaction against the target cellin vitro.Objective:The aim of this study was to obtain solutions with high dissolved oxygen levels in cultivated wells with perceiving oxygen environment and photosensitizer bleaching for photosensitization reaction studies.Materials and methods:We used a 10-mm-wide optical cell cuvette with a 1-mm optical path length as the well. A red blood cell (RBC) suspension with a hematocrit level of 0.625% was employed as the optical sample. The photosensitizer talaporfin sodium was added to a concentration of 30 μg/mL. The optical sample was irradiated by a 663-nm diode laser at 120 mW/cm2, for a total radiant exposure of 0–20 J/cm2, to induce a photosensitization reaction. Absorption spectra of the samples in the range of 475–700 nm were measured before and after each irradiation condition. Visible spectroscopy was selected to distinguish between the major three hemoglobin (Hb) types: oxygenated Hb, deoxygenated Hb, and met Hb. Also, this wavelength range was selected to investigate photobleaching using the Q band absorption peak. Each Hb concentration was estimated using a multiple regression analysis applied to the obtained absorption spectra.Results:The relationship between oxygen saturation and the absorption peak in the Q band from the talaporfin sodium dynamics with increasing radiant exposure was revealed by our method with approximately twofold oxygen-dissolved solution.Conclusions:We could perceive the oxygen environment and the photosensitization reaction progression simultaneously with increasing dissolved oxygen by adding RBCs to the cell medium and measuring the absorption spectrum of it.