PCNA appears in two populations of slow and fast diffusion with a constant ratio throughout S-phase in replicating mammalian cells
PCNA appears in two populations of slow and fast diffusion with a constant ratio throughout S-phase in replicating mammalian cells
复制标题
在复制哺乳动物细胞的整个 S 期中，PCNA 出现在慢扩散和快扩散的两个群体中，比例恒定
作者：
Patrick J. M. Zessin;A. Sporbert;M. Heilemann
DNA复制是细胞分裂之前的一个基本细胞过程。增殖细胞核抗原（PCNA）是一种中心支架蛋白，通过招募许多复制机制所必需的因子来协调DNA复制。我们使用单粒子跟踪结合光激活定位显微镜（sptPALM）研究了PCNA在活的哺乳动物细胞中的迁移，发现了两个群体。第一个群体只存在于DNA复制活跃的细胞中，扩散缓慢，位于复制病灶。第二种群表现出快速扩散，代表未结合的PCNA不参与DNA复制的核质池。这两个种群的比例在s期的不同阶段保持不变。两个种群中的一小部分分子表现出空间受限的流动性。我们确定了13%的慢扩散PCNA分子的探测半径为~100 nm， 46%的快扩散PCNA分子的探测半径为~600 nm。
DNA replication is a fundamental cellular process that precedes cell division. Proliferating cell nuclear antigen (PCNA) is a central scaffold protein that orchestrates DNA replication by recruiting many factors essential for the replication machinery. We studied the mobility of PCNA in live mammalian cells using single-particle tracking in combination with photoactivated-localization microscopy (sptPALM) and found two populations. The first population which is only present in cells with active DNA replication, showed slow diffusion and was found to be located in replication foci. The second population showed fast diffusion and represents the nucleoplasmic pool of unbound PCNA not involved in DNA replication. The ratio of these two populations remained constant throughout different stages of S-phase. A fraction of molecules in both populations showed spatially constrained mobility. We determined an exploration radius of ~100 nm for 13% of the slow-diffusing PCNA molecules and of ~600 nm for 46% of the fast-diffusing PCNA molecules.
影响因子：
4
作者：
A. Visser;F. Jaunin;S. Fakan;J. Aten
通讯作者：
A. Visser;F. Jaunin;S. Fakan;J. Aten
DOI：
10.1016/s0021-9258(18)80079-5
发表时间：
1989-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
G. Morris;M. Mathews
通讯作者：
G. Morris;M. Mathews