Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two-dimensional capillary liquid chromatography quadrupole time-of-flight mass spectrometry
Peptidomic analysis of the larval Drosophila melanogaster central nervous system by two-dimensional capillary liquid chromatography quadrupole time-of-flight mass spectrometry
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DOI：
10.1002/jms.744
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发表时间：
2005-02-01
影响因子：
2.3
通讯作者：
Schoofs, L
中科院分区：
文献类型：
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作者：
Baggerman, G;Boonen, K;Schoofs, L
肽是动物中发现的最大一类信号分子。然而，在大多数蛋白质组学研究中，肽被忽视了，因为它们实际上是从迷宫中掉出来的。类似于蛋白质组学技术，其中分析在细胞或组织中表达的所有蛋白质，肽组方法旨在同时可视化和鉴定细胞或组织的整个肽组，即具有其翻译后修饰的所有表达的肽。在本文中，我们描述了使用二维毛细管液相色谱/四极杆飞行时间串联质谱（LC/Q-TOF-MS/MS）分析幼虫果蝇中枢神经系统。仅使用50只幼虫果蝇的中枢神经系统作为起始材料，我们在一次分析中鉴定了38种肽，其中20种在先前的研究中未检测到，该研究报告了相同组织的一维毛细管LC/MS/MS分析。在38个测序的肽中，一些来自前体，例如在第一次研究中完全遗漏的速激肽和IFamide前体。这清楚地表明，二维毛细管LC方法增强了肽组分析的覆盖范围。版权所有（C）2005约翰威利父子有限公司。
Peptides are the largest class of signalling molecules found in animals. Nevertheless, in most proteomic studies peptides are overlooked since they literally fall through the mazes of the net. In analogy with proteomics technology, where all proteins expressed in a cell or tissue are analyzed, the peptidomic approach aims at the simultaneous visualization and identification of the whole pepticlome of a cell or tissue, i.e. all expressed peptides with their post-translational modifications. In this paper we describe the analysis of the larval fruit fly central nervous system using two-dimensional capillary liquid chromatography/quadrupole time-of-flight tandem mass spectrometry (LC/Q-TOF-MS/MS. Using the central nervous systems of only 50 larval Drosophila as starting material, we identified 38 peptides in a single analysis, 20 of which were not detected in a previous study that reported on the one-dimensional capillary LC/MS/MS analysis of the same tissue. Among the 38 sequenced peptides, some originate from precursors, such as the tachykinin and the IFamide precursor that were entirely missed in the first study. This clearly demonstrates that the two-dimensional capillary LC approach enhances the coverage of the peptidomic analysis. Copyright (C) 2005 John Wiley Sons, Ltd.