Preanalytical Stability of Antibodies to Pathogenic Antigens
Preanalytical Stability of Antibodies to Pathogenic Antigens
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DOI：
10.1158/1055-9965.epi-17-0170
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发表时间：
2017-08-01
影响因子：
3.8
通讯作者：
Sitas, Freddy
中科院分区：
文献类型：
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作者：
Hodgkinson, Verity S.;Egger, Sam;Sitas, Freddy
背景资料：针对病原体表位的抗体的血清学检测是研究感染与疾病之间关系的有价值的工具。本研究全面评估了分析前变异对血液样本处理延迟、预处理储存温度和真空采血管类型引起的选定抗原组抗体血清阳性的影响。方法：我们评估了从29名志愿者中采集的四种不同类型的外周血[乙二胺四乙酸（EDTA），柠檬酸盐葡萄糖（ACD），肝素锂（LH），血清分离管（SST）]，并在处理前在4 ℃或室温下储存0、1、2、3、4、5和6天。多重血清学用于确定对来自人乳头瘤病毒、人多瘤病毒、EB病毒和幽门螺杆菌的35种抗原的抗体反应性。Cohen的kappa统计量被用来衡量暴露于标准和非标准的临床实践conditions.Results之间的样品血清阳性状态的协议：对于样品处理没有延迟，kappa是不相关的存储温度（P值范围0.23至0.95）或vacutainer类型（P值范围，0.35-0.89）。对于任何真空罐型储存温度组合，Kappa值都没有随着加工延迟的增加而显著下降（P斜率范围，0.06-1.00）。结论：在处理前，可以从储存在SST、EDTA、ACD或LH真空采血管中的样品中可靠地测量来自各种致病性传染原的表位的抗体，储存温度为室温或4 ℃，最长可达6天。血清学检测对几种分析前选择具有稳健性。这些发现对于从偏远地区招募参与者的流行病学研究特别重要，因为这些地区的样本暴露于分析前条件可能差异很大。
Background: Serologic testing for antibodies against epitopes from pathogens is a valuable tool for investigating the relationship between infection and disease. This study comprehensively evaluates the impact of preanalytic variation on antibody seropositivities to a selected set of antigens arising from delays in processing of blood samples, preprocessing storage temperature, and vacutainer type.Methods: We assessed peripheral blood collected from 29 volunteers in four different Vacutainer types [ethylenediamino-etetraacetic acid (EDTA), acid-citrate-dextrose (ACD), lithium heparin (LH), serum separator tubes (SST)], and stored at 4 degrees C or room temperature for 0, 1, 2, 3, 4, 5, and 6 days before processing. Multiplex serology was used to determine antibody reactivity against 35 antigens derived from human papillomaviruses, human polyomaviruses, EpsteinBarr virus, and Helicobacter pylori. Cohen's kappa statistic was used to measure agreement on seropositivity status between samples exposed to standard and nonstandard clinical practice conditions.Results: For samples processed without delay, kappa was not associated with storage-temperature (P value range 0.23 to 0.95) or vacutainer type (P value range, 0.35-0.89). Kappa did not significantly decline with increasing delays in processing for any vacutainer-type storage temperature combination (P slope range, 0.06-1.00).Conclusions: Antibodies to epitopes from various pathogenic infectious agents can be measured reliably from samples stored in SST, EDTA, ACD, or LH vacutainers at either room temperature or 4 degrees C for up to 6 days before processing.Impact: Serologic testing is robust to several preanalytic options. These findings are particularly important for epidemiologic studies recruiting participants from remote settings where sample exposure to preanalytic conditions can vary considerably.