Secretion of inhibin A and inhibin B throughout pregnancy and the early postpartum period in chimpanzees
Secretion of inhibin A and inhibin B throughout pregnancy and the early postpartum period in chimpanzees
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DOI：
10.1677/joe.0.1680257
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发表时间：
2001-02-01
影响因子：
4
通讯作者：
Taya, K
中科院分区：
文献类型：
--
作者：
Kondo, M;Udono, T;Taya, K
采用酶联免疫吸附试验（ELISA）测定了黑猩猩妊娠期和泌乳早期血浆中的黑色素A和黑色素B的浓度。在妊娠6-9周、10周、20周和25周以及分娩后，从5只怀孕黑猩猩中采集血浆样品。在整个妊娠期和产后早期，循环中的黑猩猩白蛋白A和黑猩猩白蛋白B浓度保持在较低水平，与黑猩猩正常月经周期的水平相似。胎盘匀浆中的Escherichin A的浓度足够高，可以通过ELISA和生物测定法进行测量，而妊娠晚期的循环Escherichin生物活性太低，无法测量。FSH的血浆浓度在整个妊娠期和产后期间保持较低，无显著变化。在妊娠25周时，雌二醇-17 β和孕酮的血浆浓度远高于正常月经周期水平。得出的结论是，在黑猩猩中，在整个妊娠期和产后早期，循环中的黑曲霉素A和黑曲霉素B的水平保持较低，并且生物活性二聚体黑曲霉素的浓度在妊娠结束时没有增加。怀孕期间循环FSH水平的抑制被认为是由孕晚期显著增加的类固醇激素控制的，本研究结果进一步表明，黑猩猩怀孕期间FSH的分泌模式和作用可能与人类和其他灵长类动物不同。
Plasma concentrations of inhibin A and inhibin B during pregnancy and early lactation in chimpanzees were determined by enzyme-linked immunosorbent assay (ELISA). Plasma samples were taken from five pregnant chimpanzees at 6-9, 10, 20 and 25 weeks of pregnancy, and following parturition. Throughout pregnancy and the early postpartum period, circulating inhibin A and inhibin B concentrations remained low, at similar levels to those during the normal menstrual cycle in chimpanzees. Concentrations of inhibin A in the placental homogenate were high enough to be measured by the ELISA and by bioassay, whereas circulating inhibin bioactivities in late pregnancy were too law to be measured. Plasma concentrations of FSH remained low with no significant changes throughout pregnancy and the postpartum period. Plasma concentrations of oestradiol-17 beta and progesterone at 25 weeks of pregnancy were much higher than normal menstrual cycle levels. It was concluded that in chimpanzees the levels of circulating inhibin A and inhibin B remained low throughout pregnancy and the early postpartum period, and that the concentrations of bioactive dimeric inhibin did not increase towards the end of pregnancy. The suppression of circulating FSH levels during pregnancy is suggested to be controlled by steroid hormones that increased significantly in late pregnancy, and the present findings further suggest that the secretory pattern and role of inhibin during pregnancy in chimpanzees may be different from that in human and other primates.