A novel Brassica-rhizotron system to unravel the dynamic changes in root system architecture of oilseed rape under phosphorus deficiency
A novel Brassica-rhizotron system to unravel the dynamic changes in root system architecture of oilseed rape under phosphorus deficiency
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一种新型芸苔属-根管系统揭示缺磷条件下油菜根系结构的动态变化
DOI：
10.1093/aob/mcw083
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发表时间：
2016
期刊：
影响因子：
4.2
通讯作者：
Shi Lei
中科院分区：
文献类型：
--
作者：
Yuan Pan;Ding Guang-Da;Cai Hong-Mei;Jin Ke-Mo;Broadley Martin Roger;Xu Fang-Sen;Shi Lei
植物对缺磷的一个重要适应是通过改变根系构型（RSA）来增加从土壤中吸收磷，但基于土壤的RSA观测技术上具有挑战性，尤其是在成熟植物中。以油菜（Brassica napusL.）在整个生长周期中，在低磷和高磷土壤条件下进行。方法一种新的大型十字花科-根管系统（约。118-litre volume）来研究B.在低磷（LP）和高磷（HP）条件下，对油菜“中双11”进行全生育期的土壤培肥试验。从苗期到开花期，HP植株的总根长（TRL）、根尖数（RTN）、根长密度（RLD）、生物量和种子产量性状均比LP植株增长更快。这两个性状下降，从开花到角果期，然后略有增加，在HP植物，相反，根衰老观察到在LP植物。从聚碳酸酯板测量的RSA参数与挖掘根的分析经验一致。种子产量和地上部干重与根干重，TRL，RLD和RTN在HP和LP. ConclusionsThearica-rhizotron系统是一种有效的方法，以土壤为基础的根表型在整个生长周期。由于低磷条件下根系衰老可能发生得更早，作物缺磷可能会影响后期的水氮吸收，这对有效利用资源和优化作物产量至关重要。
Background and AimsAn important adaptation of plants to phosphorus (P) deficiency is to alter root system architecture (RSA) to increase P acquisition from the soil, but soil-based observations of RSA are technically challenging, especially in mature plants. The aim of this study was to investigate the root development and RSA of oilseed rape (Brassica napusL.) under low and high soil P conditions during an entire growth cycle.MethodsA new largeBrassica–rhizotron system (approx. 118-litre volume) was developed to study the RSA dynamics ofB. napus‘Zhongshuang11’ in soils, using top-soils supplemented with low P (LP) or high P (HP) for a full plant growth period. Total root length (TRL), root tip number (RTN), root length density (RLD), biomass and seed yield traits were measured.Key ResultsTRL and RTN increased more rapidly in HP than LP plants from seedling to flowering stages. Both traits declined from flowering to silique stages, and then increased slightly in HP plants; in contrast, root senescence was observed in LP plants. RSA parameters measured from the polycarbonate plates were empirically consistent with analyses of excavated roots. Seed yield and shoot dry weights were closely associated positively with root dry weights, TRL, RLD and RTN at both HP and LP.ConclusionsTheBrassica–rhizotron system is an effective method for soil-based root phenotyping across an entire growth cycle. Given that root senescence is likely to occur earlier under low P conditions, crop P deficiency is likely to affect late water and nitrogen uptake, which is critical for efficient resource use and optimal crop yields.