Biomimetic virus-based colourimetric sensors
Biomimetic virus-based colourimetric sensors
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DOI：
10.1038/ncomms4043
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发表时间：
2014-01-01
影响因子：
16.6
通讯作者：
Lee, Seung-Wuk
中科院分区：
文献类型：
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作者：
Oh, Jin-Woo;Chung, Woo-Jae;Lee, Seung-Wuk
自然界中的许多材料会响应刺激而改变颜色，这使得它们对于用作传感器平台具有吸引力。然而，天然材料及其合成类似物都缺乏对特定化学品的选择性，引入这种选择性仍然是一个挑战。在这里，我们报告的基因工程病毒（M13噬菌体）的自组装成目标特异性，比色生物传感器。该传感器由噬菌体束纳米结构组成，并表现出与视角无关的颜色，类似于火鸡皮肤中的胶原结构。在暴露于各种挥发性有机化学品时，这些结构迅速膨胀并发生明显的颜色变化。此外，由展示从噬菌体展示中鉴定的三硝基甲苯（TNT）结合肽基序的噬菌体组成的传感器选择性地区分低至300 ppm的TNT结构相似的化学物质。我们的可调比色传感器可用于检测各种有害有毒物质和病原体，以保护人类健康和国家安全。
Many materials in nature change colours in response to stimuli, making them attractive for use as sensor platform. However, both natural materials and their synthetic analogues lack selectivity towards specific chemicals, and introducing such selectivity remains a challenge. Here we report the self-assembly of genetically engineered viruses (M13 phage) into target-specific, colourimetric biosensors. The sensors are composed of phage-bundle nanostructures and exhibit viewing-angle independent colour, similar to collagen structures in turkey skin. On exposure to various volatile organic chemicals, the structures rapidly swell and undergo distinct colour changes. Furthermore, sensors composed of phage displaying trinitrotoluene (TNT)-binding peptide motifs identified from a phage display selectively distinguish TNT down to 300 p.p.b. over similarly structured chemicals. Our tunable, colourimetric sensors can be useful for the detection of a variety of harmful toxicants and pathogens to protect human health and national security.