LIPOPROTEIN-LIPASE MASS AND ACTIVITY IN PLASMA AND THEIR INCREASE AFTER HEPARIN ARE SEPARATE PARAMETERS WITH DIFFERENT RELATIONS TO PLASMA-LIPOPROTEINS
LIPOPROTEIN-LIPASE MASS AND ACTIVITY IN PLASMA AND THEIR INCREASE AFTER HEPARIN ARE SEPARATE PARAMETERS WITH DIFFERENT RELATIONS TO PLASMA-LIPOPROTEINS
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DOI：
10.1161/01.atv.15.8.1086
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发表时间：
1995-08-01
影响因子：
8.7
通讯作者：
OLIVECRONA, T
中科院分区：
文献类型：
--
作者：
TORNVALL, P;OLIVECRONA, G;OLIVECRONA, T
研究人员测量了 61 名 45 岁之前患有心肌梗塞的男性和 69 名年龄和性别匹配的无冠心病对照受试者的血浆中脂蛋白脂肪酶（LPL）活性和质量及其在给予肝素后的增加，以研究血浆中这些参数之间的关系以及它们与具有广泛脂蛋白和 LPL 水平的受试者血浆脂蛋白的相关性。与肝素前血浆中的LPL活性相比，存在相对大量的LPL蛋白，表明大部分循环LPL是无催化活性的。 LPL 质量和肝素后血浆中的活性（肝素后减去肝素前值）高度相关。计算出的平均比活性 (0.35 mU/ng) 在催化活性 LPL 的预期范围内。因此，肝素主要释放活性LPL。四个 LPL 参数（血浆中的质量和活性及其在肝素给药后的增加）彼此不相关：除了肝素后血浆 LPL 质量和活性外，它们与血浆脂蛋白脂质浓度表现出不同的相关性。肝素前血浆中LPL质量与患者HDL胆固醇水平呈强正相关，与VLDL甘油三酯和胆固醇浓度呈弱负相关。相反，在对照组中，肝素前体 LPL 活性与 HDL 胆固醇水平没有相关性，但与 VLDL 甘油三酯和胆固醇浓度呈弱正相关。在对照组中，肝素后血浆 LPL 活性与 HDL 胆固醇水平呈正相关，与 VLDL 甘油三酯浓度呈负相关。病例受试者与对照受试者的不同之处在于，他们具有较高的肝素前血浆 LPL 活性，并且倾向于较低的肝素后血浆 LPL 比活性。患者和对照受试者之间测得的 LPL 参数与血浆脂蛋白的不同关系以及肝素前血浆 LPL 活性的差异可能反映了患者 LPL 系统的紊乱。
Lipoprotein lipase (LPL) activity and mass in plasma and their increase after heparin administration were measured in 61 men who had suffered myocardial infarction before the age of 45 years and in 69 population-based age- and sex-matched control subjects without coronary heart disease to study the relations between these parameters in plasma and their correlations with plasma lipoproteins in subjects with a wide range Of lipoprotein and LPL levels. There was a relatively large amount of LPL protein compared with LPL activity in preheparin plasma, indicating that the majority of circulating LPL is catalytically inactive. LPL mass and activity in postheparin plasma (postheparin minus preheparin values) were highly correlated. and the calculated mean specific activity (0.35 mU/ng) was in the range expected for catalytically active LPL. Hence, heparin releases mainly active LPL. The four LPL parameters (mass and activity in plasma and their increase after heparin administration) were not related to each other: except for postheparin plasma LPL mass and activity, and they showed different correlations with plasma lipoprotein lipid concentrations. There was a strong positive correlation between LPL mass in preheparin plasma and the HDL cholesterol level as well as weak negative relations to VLDL triglyceride and cholesterol concentrations in the patients. In contrast, preheparin LPL activity showed no correlation with the HDL cholesterol level but weak positive relations to VLDL triglyceride and cholesterol concentrations in the control subjects. Postheparin plasma LPL activity related positively to the HDL cholesterol level and negatively to the VLDL triglyceride concentration in the control subjects. Case subjects differed from control subjects in that they had higher preheparin plasma LPL activity and a tendency toward lower specific activity of postheparin plasma LPL. The different relations of the measured LPL parameters to plasma lipoproteins and the difference in preheparin plasma LPL activity between patients and control subjects might reflect a disturbance of the LPL system in the patients.