Characterization of outer retinal morphology with high-speed, ultrahigh-resolution optical coherence tomography
Characterization of outer retinal morphology with high-speed, ultrahigh-resolution optical coherence tomography
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DOI：
10.1167/iovs.07-0838
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发表时间：
2008-04-01
影响因子：
4.4
通讯作者：
Fujimoto, James G.
中科院分区：
文献类型：
--
作者：
Srinivasan, Vivek J.;Monson, Bryan K.;Fujimoto, James G.
目的.利用高速超高分辨率OCT对视网膜外表面形态进行可视化、定量评价和解释。在眼科诊所中，使用3.5-β m、轴向分辨率、高速、UHR OCT原型仪器在43名正常受试者的横截面中进行视网膜成像，使用径向扫描模式（24张图像，1500次轴向扫描）。视网膜外层自动分割和测量。高清晰度成像进行了2.8 β m轴向分辨率，高速，UHR OCT研究原型仪器，可视化的外部视网膜的精细功能。外视网膜层的定量图显示出明显的差异之间的锥为主的中央凹和杆为主的旁凹和中央凹周，表明感光细胞形态可以解释高速，UHR OCT图像中的外视网膜的外观。使用高清晰度成像在外层视网膜中观察到更精细的散射带，并通过与已知解剖结构的比较进行解释。高速UHR OCT能够量化外层视网膜中的散射层。这些功能的解释，并支持在正常受试者的定量测量和比较与已知的解剖结构。厚的散射区域的外视网膜先前归因于视网膜色素上皮细胞（RPE）被证明是由不同的散射带对应的感光细胞外段尖端，RPE，和布鲁赫膜。这些结果可能会进一步了解OCT图像中的外部视网膜外观。标准测量也可能有助于未来对年龄相关性黄斑变性和其他疾病的外部视网膜变化的研究。
PURPOSE. To visualize, quantitatively assess, and interpret outer retinal morphology by using high-speed, ultrahigh-resolution ( UHR) OCT.METHODS. Retinal imaging was performed in the ophthalmic clinic in a cross-section of 43 normal subjects with a 3.5-beta m, axial-resolution, high-speed, UHR OCT prototype instrument, using a radial scan pattern ( 24 images, 1500 axial scans). Outer retinal layers were automatically segmented and measured. High-definition imaging was performed with a 2.8-beta m axial-resolution, high-speed, UHR OCT research prototype instrument, to visualize the finer features in the outer retina.RESULTS. Quantitative maps of outer retinal layers showed clear differences between the cone-dominated fovea and the rod-dominated parafovea and perifovea, indicating that photoreceptor morphology can explain the appearance of the outer retina in high-speed, UHR OCT images. Finer, scattering bands were visualized in the outer retina using high-definition imaging and were interpreted by comparison to known anatomy.CONCLUSIONS. High-speed UHR OCT enables quantification of scattering layers in the outer retina. An interpretation of these features is presented and supported by quantitative measurements in normal subjects and comparison with known anatomy. The thick scattering region of the outer retina previously attributed to the retinal pigment epithelium ( RPE) is shown to consist of distinct scattering bands corresponding to the photoreceptor outer segment tips, RPE, and Bruch's membrane. These results may advance understanding of the outer retinal appearance in OCT images. The normative measurements may also aid in future investigations of outer retinal changes in age-related macular degeneration and other diseases.