A method for rapid analysis of biofilm morphology and coverage on glass and polished and brushed stainless steel
A method for rapid analysis of biofilm morphology and coverage on glass and polished and brushed stainless steel
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DOI：
10.13031/2013.8506
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发表时间：
2002-03-01
期刊：
影响因子：
--
通讯作者：
Ali, L
中科院分区：
文献类型：
--
作者：
Lomander, A;Schreuders, P;Ali, L
开发了一种利用落射荧光显微镜和图像分析快速分析生物膜的工具。该工具允许评价整体生物膜覆盖率和生物膜斑块形态。它将提供一种定量方法来识别生物膜及其基质之间的关系以及识别受损细菌斑块的形态。覆盖百分比允许评估生物膜的生长，并将允许评估消毒剂和机械清洁方法在从食品加工表面去除生物膜方面的有效性。贴片形态可用于研究表面形态和生物膜生长之间的关系，以及用于确定表面形态、生物膜形状和消毒剂有效性。利用大肠杆菌生物膜对软件进行了测试和验证。coliK 12在玻璃上，抛光316不锈钢，和拉丝316不锈钢。生物膜用碘化丙啶和SYTO-16染色。在对整体覆盖率和生物膜斑块形态进行数字图像分析后，发现活生物膜或总生物膜的百分比与基质材料无关。然而，个体生物膜斑块的绝对面积根据基质材料而变化。这些领域也随着时间的推移而发生变化。研究了生物膜斑块的圆形度。在研究结束时，该组中大多数生物膜斑块的形状接近圆形。
A tool for the rapid analysis of biofilms, using epifluorescence microscopy and image analysis was developed. The tool allows the evaluation of overall biofilm coverage and biofilm patch morphology. It will provide a quantitative method for identifying relationships between biofilms and their substrates and for identifying the morphology of patches of damaged bacteria. The coverage percentage allows evaluation of the growth of biofilms and will allow the evaluation of the effectiveness of sanitizers and mechanical cleaning methods in removing biofilms from food processing surfaces. The patch morphology is useful for investigating the relationships between surface morphology and biofilm growth and for determining the surface morphology, biofilm shape, and sanitizer effectiveness. The software was tested and validated using biofilms of E. coli K12 on glass, polished 316 stainless steel, and brushed 316 stainless steel. The biofilms were stained with both propidium iodide and SYTO-16. After peforming digital image analysis of both overall coverage and biofilm patch morphometries, it was found that the percentages of living or total biofilm were independent of the substrate material. However, the absolute area of individual biofilm patches varied depending on the substrate material. These areas also showed changes over time. The circularity of the biofilm patches was investigated. At the end of the studies, the shape of most of the biofilm patches in this group was nearly circular.