A sequential extraction procedure reveals that water management affects soil nematode communities in paddy fields
A sequential extraction procedure reveals that water management affects soil nematode communities in paddy fields
复制标题
连续提取程序揭示水管理影响稻田土壤线虫群落
DOI：
10.1016/j.apsoil.2008.05.001
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发表时间：
2008-10-01
影响因子：
4.8
通讯作者：
Hu, Feng
中科院分区：
文献类型：
--
作者：
Liu, Manqiang;Chen, Xiaoyun;Hu, Feng
在季节性干旱非常严重的亚热带地区，用较少的水维持水稻高产的管理效果越来越受到重视。为了测试水分管理是否影响土壤生态过程，我们比较了晚稻水分管理处理后稻田土壤线虫群落的变化。处理为连续淹水水稻(CF)、不连续淹水水稻(ZM)和稻草覆盖(M)。采用Baermann提取(Baermann)和离心糖浮选(离心法)相结合的顺序提取程序来提取整个线虫群落。根据线虫丰度、属组成、营养类群和其他生态指标(Margalef丰富度指数、Shannon指数、成熟度指数、丰富度指数和结构指数)的分析，水分管理对线虫群落有显著影响，在非连续淹水条件下，特别是与稻草覆盖(M)相结合，促进了线虫的发育。覆盖处理的线虫群落具有较高的丰富度、丰富度和结构，表明生物多样性和生物相互作用得到改善。我们假设土壤小气候和可利用的食物资源受到水管理的影响，并直接或间接地影响不同线虫群落的建立。我们认为，单纯使用Baermann方法可能会扭曲旨在测试自然或人为影响线虫的研究。尽管顺序提取程序中的Baermann步骤提供了关于线虫对土壤处理的反应的有价值的信息，但这不足以检测对整个线虫群落的影响。我们的结果表明，顺序提取程序是获得整个线虫群落的全面信息的有效工具，这两个步骤都提供了补充的生态相关信息，即土壤生态系统如何受到人类管理的影响。(C)2008爱思唯尔B.V.保留所有权利。
The effects of management in maintaining high rice yield with less water have received increasing attention in sub-tropical regions where seasonal drought is very severe. To test whether water management affects soil ecological process we compared the soil nematode communities in paddy field soils following water management treatments imposed during late rice cropping. The treatments were continuously flooded rice cultivation (CF), noncontinuously flooded rice cultivations without (ZM) and with (M) rice straw mulching. A sequential extraction procedure involving Baermann extraction (Baermann) followed by centrifugal sugar flotation (centrifugation) was used to extract the whole nematode community. Based on the analysis of nematode abundance, genera composition, trophic groups and other ecological indices (Margalef richness index, Shannon index, maturity index, enrichment index and structure index), water management significantly affected the nematode community, with the non-continuously flooded condition, in particular combination with rice straw mulching (M), stimulating nematode development. There was a high abundance, richness, and structure in the nematode community in mulched treatments, indicating improved biodiversity and biological interactions. We hypothesize that soil microclimate and available food resources are affected by water management and directly or indirectly influence the establishment of different nematode communities. We suggested that studies aiming to test natural or anthropogenic influences on nematodes might be distorted by sole use of the Baermann method. Although the Baermann step in a sequential extraction procedure gave valuable information on nematode responses to soil treatments, it was not sufficient to detect effects on the whole nematode community. Our results demonstrate that the sequential extraction procedure is an effective tool to obtain comprehensive information on the whole nematode community, with both steps providing complementary ecologically relevant information, on how the soil ecosystem is affected by human management. (C) 2008 Elsevier B.V. All rights reserved.