Development of an enzyme-linked immunosorbent assay (ELISA) for natamycin residues in foods based on a specific monoclonal antibody
Development of an enzyme-linked immunosorbent assay (ELISA) for natamycin residues in foods based on a specific monoclonal antibody
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DOI：
10.1039/c5ay00404g
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发表时间：
2015-01-01
影响因子：
3.1
通讯作者：
Xu, Chuanlai
中科院分区：
文献类型：
--
作者：
Chen, Yanni;Kong, Dezhao;Xu, Chuanlai
建立了一种针对纳他霉素（Nata）的单克隆抗体（mAb）的间接竞争性酶联免疫吸附试验（ic-ELISA），用于检测牛奶、果汁、酸奶和奶酪样品中的纳他霉素（Nata）。ic-ELISA的工作范围为0.64 ~ 4.46 μ g -1， IC50值为1.69 μ g -1。加样回收率分别为103-121%、103-121%、84-114%、89-108%。结果表明，该方法可有效地用于食品中Nata的分析。
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) was developed based on a sensitive and specific monoclonal antibody (mAb) against natamycin (Nata) for Nata detection in milk, juice, yoghurt, and cheese samples. The working range of ic-ELISA was 0.64-4.46 mu g L-1 with an IC50 value of 1.69 mu g L-1. The average recoveries of milk, juice, yoghurt, and cheese samples spiked with Nata were 103-121%, 103-121%, 84-114%, and 89-108%, respectively. The results indicated that ic-ELISA can be effectively applied for Nata analysis in these food products.