Phenytoin (dilantin)‐induced cleft lip and palate in a/j mice: A scanning and transmission electron microscopic study
Phenytoin (dilantin)‐induced cleft lip and palate in a/j mice: A scanning and transmission electron microscopic study
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苯妥英（苯妥英钠）诱导的 a/j 小鼠唇裂和腭裂：扫描和透射电子显微镜研究
DOI：
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发表时间：
1979
期刊：
影响因子：
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通讯作者：
D. Dorgan
中科院分区：
文献类型：
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作者：
K. Sulik;M. C. Johnston;L. J. Ambrose;D. Dorgan
妊娠第10天母体腹腔内给予A/J小鼠75 mg/kg培妥英后，唇腭裂（CLP）的发生率较高，与侧鼻突的严重缩小相关。该区域的扫描和透射电镜分析表明，处理组与对照组第11天（7尾体节）胚胎中表面上皮下方的间充质细胞形态发生显著变化：苯妥英处理组胚胎中未发育或不存在长的分支细胞突起，其形成致密的网状结构，似乎与对照胚胎中的上皮基底层相互作用。这些细胞过程的超微结构形态的描述和它们可能在正常发展的重要性进行了讨论。扫描电镜观察常由苯妥英治疗引起的不完全唇裂表明，Simonart带是由外侧和内侧鼻突融合形成的，与上颌突和内侧鼻突缺乏融合有关。
High incidences of cleft lip and palate (CLP) Produced by maternal intraperitoneal administration of 75 mg/kg pehnytoin on gestational day 10 to A/J mice are associated with asevere size reduction in the lateral nasal process. Scanning and transmission EM analyses of this region demonstrate a marked change in the morphology of the mesenchymal cells underlying the surface epithelium in treated versus control day 11 (7‐tail somite) embryos: long, branching cellular processes, which form a dense meshwork that appears to interact with the epithelial basement lamina in control embryos are undeveloped or absent in phenytoin‐treated embryos. The ultrastructural morphology of these cell processes is described and their possible importance in normal development is discussed. Scanning EM observations of incomplete clefts of the lip which frequently result from phenytoin treatment indicate that Simonart's bands form from fusionof the lateral and medial nasal processes in association with a lack of fusion of the maxillary process with the medial nasal process.