Stromal progesterone receptors mediate the inhibitory effects of progesterone on estrogen-induced uterine epithelial cell deoxyribonucleic acid synthesis
Stromal progesterone receptors mediate the inhibitory effects of progesterone on estrogen-induced uterine epithelial cell deoxyribonucleic acid synthesis
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DOI：
10.1210/en.139.11.4708
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发表时间：
1998-11-01
期刊：
影响因子：
4.8
通讯作者：
Cunha, GR
中科院分区：
文献类型：
--
作者：
Kurita, T;Young, P;Cunha, GR
通过分析野生型（wt）和PR敲除（PRKO）小鼠子宫间质（S）和上皮（E）制备的4种组织重组体：wt-S + wt-E、PRKO-S + PRKO-E，探讨上皮和基质孕酮（P）受体（PR）在P调节子宫上皮DNA合成中的作用。 wt-S + PRKO-E，和 PRKO-S + wt-E。 17-β 雌二醇 (E-2) 刺激所有四种类型的组织重组体中的 DNA 合成。另一方面，P 仅在用野生型（PR 阳性）基质（wt-S + wt-E 或 wt-S + PRKO-E）制备的组织重组体中抑制 E-2 诱导的 DNA 合成，但不抑制敲除（PR 阴性）基质（PRKO-S + wt-E 或 PRKO-S + PRKO-E）。这些结果清楚地表明P对子宫上皮DNA合成的抑制作用是由基质PR介导的。上皮 PR 对于 P 抑制 E-2 诱导的上皮 DNA 合成既不是必要的也不是充分的。
The role of epithelial and stromal progesterone (P) receptors(PR) in the regulation of uterine epithelial DNA synthesis by P was investigated by analyzing the four types of tissue recombinants prepared with uterine stroma (S) and epithelium (E) from wild-type (wt) and PR knockout (PRKO)mice:wt-S + wt-E, PRKO-S + PRKO-E, wt-S + PRKO-E, and PRKO-S + wt-E. 17-beta estradiol (E-2) stimulated DNA synthesis in all four types of tissue recombinants. On the other hand, P inhibited E-2-indueed DNA synthesis only in tissue recombinants prepared with wild-type (PR-positive) stroma (wt-S + wt-E or wt-S + PRKO-E) but not knockout (PR-negative) stroma (PRKO-S + wt-E Or PRKO-S + PRKO-E). These results clearly demonstrate that the inhibitory effect of P on uterine epithelial DNA synthesis is mediated by stromal PR. Epithelial PR is neither necessary nor sufficient for P inhibition of E-2-induced epithelial DNA synthesis.