Cloning and characterization of porcine resistin gene
Cloning and characterization of porcine resistin gene
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DOI：
10.1016/j.domaniend.2005.06.003
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发表时间：
2006-02-01
影响因子：
2.1
通讯作者：
Yang, ZQ
中科院分区：
文献类型：
--
作者：
Dai, MH;Xia, T;Yang, ZQ
抵抗素是抵抗素样分子（RELM）的一员，是啮齿动物成熟脂肪细胞和人类白细胞分泌的一种激素。我们现在报告的全长的猪cDNAs和基因的克隆和鉴定。序列分析结果表明，该基因的开放阅读框为330 bp，编码109个氨基酸，分子量为12 kDa。推导出的氨基酸序列与人源cDNA的同源性为75.2%。该基因由4个外显子组成，与人的外显子结构完全相同。采用半定量RT-PCR方法检测猪Bcln mRNA的组织分布。抵抗素基因在猪白细胞中表达最高，在脂肪组织中表达较低。免疫印迹法在猪血清中检测到抵抗素蛋白，其在血清中以二聚体和三聚体形式存在。我们提供的第一个证据表明，在猪白细胞中大量表达，并有一个类似的表达模式，在人的cDNAn mRNA和蛋白质。提示猪可能是研究胰岛素抵抗的理想动物模型。(c)2005年爱思唯尔公司All rights reserved.
Resistin is a member of resistin-like molecules (RELMs) and a hormone secreted from mature adipocytes in rodents and leukocytes in human. We now report the cloning and characterization of the full-length porcine resistin cDNA and gene. Sequence analysis indicated that the pig resistin cDNA sequence had an open reading frame of 330 bp encoding a 12 kDa protein of 109 amino acids. The deduced amino acid sequence showed 75.2% identity to the human resistin. The porcine resistin gene was composed of four exons and had exactly the same exon structure as the human resistin gene. The tissue distribution of porcine resistin mRNA was assessed by semi-quantitative RT-PCR. Resistin gene expression was the highest in porcine leukocytes and low in adipose tissue. Resistin protein could be detected in porcine serum by western blotting and it circulated in serum as dimers and trimers. We provided the first evidence that resistin was abundantly expressed in porcine leukocytes and had an expression pattern similar to that in human resistin mRNA and protein. This suggests that the pig may be a suitable animal model for studying the function of resistin in human insulin resistance. (c) 2005 Elsevier Inc. All rights reserved.