SUBCUTANEOUS GLUCOSE-CONCENTRATION IN HUMANS - REAL ESTIMATION AND CONTINUOUS MONITORING
SUBCUTANEOUS GLUCOSE-CONCENTRATION IN HUMANS - REAL ESTIMATION AND CONTINUOUS MONITORING
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DOI：
10.2337/diacare.18.9.1266
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发表时间：
1995-09-01
期刊：
影响因子：
16.2
通讯作者：
PFEIFFER, EF
中科院分区：
文献类型：
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作者：
STERNBERG, F;MEYERHOFF, C;PFEIFFER, EF
目的-要确定健康志愿者的真实的皮下葡萄糖浓度，以帮助开发新的校准方法皮下glucosensors.Research设计和方法-我们开发了一种新的方法来估计的真实的皮下葡萄糖浓度的基础上再循环的磷酸盐缓冲盐水（PBS）在微透析探头插入到皮下组织。组织葡萄糖扩散到探针中，直到实现微透析探针外部和内部的葡萄糖浓度之间的完全平衡。随后，在体外评估再循环PBS的葡萄糖含量。我们将该方法应用于10名健康志愿者在空腹状态下和在高血糖钳夹期间。此外，我们监测皮下葡萄糖与酶安培葡萄糖传感器结合微透析probes.RESULTS -皮下葡萄糖浓度测定的再循环方法是72 +/- 6和78 +/- 6%的血糖测量空腹状态和高血糖钳夹期间，分别。另一方面，葡萄糖传感器的信号与血糖显着相关。结论再循环方法估计的真实的皮下葡萄糖浓度，开辟了新的校准程序，为皮下葡萄糖传感器。然而，仍然缺乏适当的校准程序。
OBJECTIVE - To determine the real subcutaneous glucose concentration in healthy volunteers to help in the development of new calibration methods for subcutaneous glucosensors.RESEARCH DESIGN AND METHODS - We developed a new method to estimate the real subcutaneous glucose concentration based on the recirculation of phosphate-buffered saline (PBS) in a microdialysis probe inserted into the subcutaneous tissue. Tissue glucose diffuses into the probe until complete equilibration between the glucose concentration outside and inside the microdialysis probe is achieved. Later, the glucose content of the recirculated PBS is assessed in vitro. We applied the method in 10 healthy volunteers under fasting state and during a hyperglycemic clamp. in addition, we monitored the subcutaneous glucose with an enzymatic-amperometric glucosensor combined with a microdialysis probe.RESULTS - The subcutaneous glucose concentration measured by the recirculation method was 72 +/- 6 and 78 +/- 6% of the blood glucose measured in the fasting state and during the hyperglycemic clamps, respectively. On the other hand, the glucosensor's signal correlated significantly with the blood glucose.CONCLUSION The recirculation method estimated the real subcutaneous glucose concentration, opening the way to develop new calibration procedures for subcutaneous glucosensors. However, a suitable calibration procedure is still lacking.