High Expression of SOX2 and OCT4 Indicates Radiation Resistance and an Independent Negative Prognosis in Cervical Squamous Cell Carcinoma
High Expression of SOX2 and OCT4 Indicates Radiation Resistance and an Independent Negative Prognosis in Cervical Squamous Cell Carcinoma
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SOX2和OCT4的高表达表明宫颈鳞状细胞癌的放射抵抗和独立的阴性预后
DOI：
10.1369/0022155414532654
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发表时间：
2014-07-01
影响因子：
3.2
通讯作者：
Chen, Xiang
中科院分区：
文献类型：
--
作者：
Shen, Liangfang;Huang, Xinqiong;Chen, Xiang
放射治疗（RT）作为术前或术后的辅助或主要治疗是局部晚期宫颈癌最常见的管理方式。肿瘤细胞的放射抗性仍然是一个主要的治疗问题。因此，我们旨在探索干细胞生物标志物SOX 2和OCT 4蛋白是否可用于预测局部晚期宫颈鳞状细胞癌（LACSCC）患者的放射抵抗。根据无进展生存期（PFS）将这132例患者分为两组（放射抵抗组和放射敏感组）。使用预处理的石蜡包埋组织，我们评估SOX 2和OCT 4的表达使用免疫组化染色。放射抵抗组中SOX 2和OCT 4的过表达百分比明显高于放射敏感组（分别为p<0.001和p <0.001）。SOX 2和OCT 4高表达患者的PFS短于低表达患者。我们的研究表明，肿瘤细胞中SOX 2和OCT 4的表达表明对放射治疗的抵抗，这两个因素是LACSCC患者生存不良的重要预测因素（风险比[95% CI]分别为2.294 [1.013，5.195]和2.300 [1.050，5.037];分别为p=0.046和p=0.037）。
Radiotherapy (RT) as a preoperative or postoperative adjuvant or primary treatment is the most common management modality for locally advanced cervical cancer. Radioresistance of tumor cells remains a major therapeutic problem. Consequently, we aimed to explore if the stem cell biomarkers SOX2 and OCT4 protein could be used to predict radioresistance in patients with locally advanced cervical squamous cell carcinoma (LACSCC). These 132 patients were divided into two groups (radiation-resistant and radiation-sensitive groups) according to progress-free survival (PFS). Using pretreatment paraffin-embedded tissues, we evaluated SOX2 and OCT4 expression using immunohistochemical staining. The percentage of overexpression of SOX2 and OCT4 in the radiation-resistant group was much higher than that in the radiation-sensitive group (p<0.001 and p <0.001, respectively). The patients with high expression of SOX2 and OCT4 showed a shorter PFS than those with low expression. Our study suggests that the expression of SOX2 and OCT4 in tumor cells indicates resistance to radiotherapy and that these two factors were important predictors of poor survival in patients with LACSCC (hazard ratio [95% CI], 2.294 [1.013, 5.195] and 2.300 [1.050, 5.037], respectively; p=0.046 and p=0.037, respectively).