Expression of p40/Epstein-Barr virus nuclear antigen 1 binding protein 2.
Expression of p40/Epstein-Barr virus nuclear antigen 1 binding protein 2.
复制标题
p40/Epstein-Barr 病毒核抗原 1 结合蛋白 2 的表达。
DOI：
10.1006/bbrc.2001.4780
复制
发表时间：
2001
影响因子：
3.1
通讯作者：
Valdez,BC
中科院分区：
文献类型：
--
作者：
Henning,D;Valdez,BC
核仁蛋白p40/EBP2是一种与EB病毒核抗原1(EBNA1)相互作用以维持EB病毒(EBV)上体的增殖相关抗原。酵母p40/EBP2在将27S-A加工成27S-B核糖体RNA的过程中发挥作用。本研究报道了青蛙、鸡、猪、大鼠、小鼠、牛和人的p40/EBP2基因的氨基酸序列高度进化保守。P40/EBP2在人体组织中广泛表达。与所检测的其他细胞系相比，它在髓系白血病K-562中高表达。人类p40/EBP2基因位于1号染色体上，由9个外显子和8个内含子组成。最小的启动子区域位于ATG起始密码子的上游300个核苷酸，前面是一个富含嘧啶的区域。这两个区域包含8个Sp1和4个c-ETS-1推测的结合位点。对p40/EBP2基因及其启动子区域的分析将有助于研究其在EBV感染和未感染细胞中的表达调控。
Nucleolar protein p40/EBP2 is a proliferation-associated antigen that interacts with Epstein–Barr virus nuclear antigen 1 (EBNA1) to maintain the Epstein–Barr virus (EBV) episomes. The yeast p40/EBP2 functions in the processing of 27S-A into 27S-B ribosomal RNA. The present study reports high evolutionary conservation of the cDNA-derived amino acid sequences of p40/EBP2 from frog, chicken, pig, rat, mouse, bovine, and human. p40/EBP2 is ubiquitously expressed in human tissues. It is highly expressed in myelogenous leukemia K-562 compared to other cell lines tested. The human p40/EBP2 gene is located in chromosome 1 with nine exons and eight introns. The minimal promoter region resides 300 nucleotides upstream of a putative ATG initiation codon preceded by a pyrimidine-rich region. These two regions contain eight Sp1 and four c-Ets-1 putative binding sites. Analysis of the p40/EBP2 gene and its promoter region will facilitate studies on the regulation of its expression in EBV-infected and noninfected cells.