Development and aging of the articular cartilage of the rabbit knee joint: Distribution of biglycan, decorin, and matrilin-1
Development and aging of the articular cartilage of the rabbit knee joint: Distribution of biglycan, decorin, and matrilin-1
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DOI：
10.1177/002215549904701212
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发表时间：
1999-12-01
影响因子：
3.2
通讯作者：
Ashhurst, DE
中科院分区：
文献类型：
--
作者：
Kavanagh, E;Ashhurst, DE
本研究测定了兔膝关节软骨发育和老化过程中小分子蛋白聚糖、双糖蛋白聚糖、核心蛋白聚糖和糖蛋白matrilin-1（软骨基质蛋白）的分布。在空化之前，间带和邻近的骺软骨的基质不包含双糖链或核心蛋白聚糖，但一些软骨细胞表达它们的mRNA。Matrilin-1仅存在于更深的骺软骨中。空化后，在假定的关节软骨中检测到双糖蛋白聚糖和核心蛋白聚糖，但没有matrilin-1。所有这些都存在于下面的骺软骨，关节在新生儿中，骺软骨骨化，关节软骨成为一个离散的层。双糖蛋白聚糖和核心蛋白聚糖在关节软骨中积累，但matrilin-1仍然局限于残留的骺软骨。在成虫中，双糖链蛋白聚糖和核心蛋白聚糖的分布是高度可变的。核心蛋白聚糖倾向于局限于中心区域; matrilin-1不存在。结果表明，关节软骨和骺软骨与早期发育阶段不同。骺软骨可以通过其具有matrilin-1来鉴定。骺软骨在发育过程中被移除，留下关节软骨。讨论了核心蛋白聚糖和基质蛋白-1的分布与纤维状胶原的关系。
We determined the distributions of the small proteoglycans biglycan and decorin and the glycoprotein matrilin-1 (cartilage matrix protein) during development and aging of articular cartilage in the rabbit knee joint. Before cavitation, the matrices of the interzone and the adjacent epiphyseal cartilage do not contain biglycan or decorin, but some chondrocytes express their mRNAs. Matrilin-1 is found only in the deeper epiphyseal cartilage. After cavitation, biglycan and decorin are detected in the presumptive articular cartilage, but there is no matrilin-1. All are present in the underlying epiphyseal cartilage, articular In the neonate, the epiphyseal cartilage is ossified and the articular cartilage becomes a discrete layer. Biglycan and decorin accumulate in the articular cartilage, but matrilin-1 remains confined to the residual epiphyseal cartilage. In the adult, the distributions of biglycan and decorin are highly variable. Decorin tends to be confined to the central region; matrilin-1 is absent. The findings indicate that the articular and epiphyseal cartilages are different from the earliest developmental stages. The epiphyseal cartilage can be identified by its possession of matrilin-1. Epiphyseal cartilage is removed during development to leave the articular cartilage. The relationships between the distributions of decorin and matrilin-1 and the fibrillar collagens are discussed.