A More Sensitive Radioimmunoassay for Neuron‐Specific Enolase Suitable for Cerebrospinal Fluid Determinations
A More Sensitive Radioimmunoassay for Neuron‐Specific Enolase Suitable for Cerebrospinal Fluid Determinations
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DOI：
10.1111/j.1471-4159.1981.tb01704.x
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发表时间：
1981-03
影响因子：
4.7
通讯作者：
A. Parma;P. Marangos;F. Goodwin
中科院分区：
文献类型：
--
作者：
A. Parma;P. Marangos;F. Goodwin
摘要：神经元特异性烯醇化酶（NSE）和非神经元烯醇化酶（NNE）已被证明分别是高度特异性的神经元和神经胶质产物，因此可用作脊椎动物中枢神经系统中两种主要细胞类型的生化标记物。描述了一种用于人NSE（NSE-H）的碘化放射免疫测定（RIA）程序，其灵敏度比先前可用的氚化测定高约50倍。该测定法每次测定能够检测100 pg NSE-H。人脑脊液（CSF）中的NSE水平以前用氚化RIA检测不到，现在可以很容易地测量，并已显示约为2 ng/ml CSF。此外，用新描述的测定程序对更浓缩的脑组织提取物获得的结果与氚化RIA相当。碘化NSE RIA还显示能够准确检测人CSF中NSE的添加量，表明该测定法在确定CSF中NSE水平升高中的潜在临床有用性。
Abstract: Neuron‐specific enolase (NSE) and non‐neuronal enolase (NNE) have been shown to be highly specific neuronal and glial products respectively and are therefore useful as biochemical markers of the two major cell types in the vertebrate central nervous system. An iodinated radioimmunoassay (RIA) procedure for human NSE (NSE‐H) with approximately 50‐fold greater sensitivity than the previously available tritiated assay is described. This assay is capable of detecting 100 pg of NSE‐H per assay. NSE levels in human cerebrospinal fluid (CSF) which were previously undetectable with the tritiated RIA are now easily measured and have been shown to be approximately 2 ng/ml of CSF. Furthermore, results obtained with the newly described assay procedure on more concentrated brain tissue extracts are comparable to the tritiated RIA. The iodinated NSE RIA is also shown to be capable of accurately detecting added amounts of NSE in human CSF, indicating the potential clinical usefulness of this assay in determining elevated levels of NSE in CSF.