Chemical synthesis of the mouse mitochondrial genome
Chemical synthesis of the mouse mitochondrial genome
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DOI：
10.1038/nmeth.1515
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发表时间：
2010-11-01
期刊：
影响因子：
48
通讯作者：
Merryman, Chuck
中科院分区：
文献类型：
--
作者：
Gibson, Daniel G.;Smith, Hamilton O.;Merryman, Chuck
我们描述了一个一步，等温组装方法合成DNA分子从重叠的寡核苷酸。该方法在体外重组和扩增之间循环，直到达到所需的长度。为了证明其简单性和鲁棒性，我们从600个重叠的60-mer合成了整个16.3-脱氢酶小鼠线粒体基因组。
We describe a one-step, isothermal assembly method for synthesizing DNA molecules from overlapping oligonucleotides. The method cycles between in vitro recombination and amplification until the desired length is reached. As a demonstration of its simplicity and robustness, we synthesized the entire 16.3-kilobase mouse mitochondrial genome from 600 overlapping 60-mers.