T-CELL NECESSITY IN PATHOGENESIS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS IN MICE
T-CELL NECESSITY IN PATHOGENESIS OF EXPERIMENTAL AUTOIMMUNE ENCEPHALOMYELITIS IN MICE
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DOI：
10.1002/eji.1830060912
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发表时间：
1976-01-01
影响因子：
5.4
通讯作者：
MACKAY, IR
中科院分区：
文献类型：
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作者：
BERNARD, CCA;LEYDON, J;MACKAY, IR
实验性自身免疫性脑脊髓炎（EAE）的细胞转移是由适当免疫供体的淋巴结和脾细胞进行的。免疫血清没有转移这种自身免疫性疾病，也没有任何促进或抑制作用的淋巴细胞转移EAE的能力。EAE的转移在正常小鼠，轻度照射（350拉德）和致死照射（850拉德）和骨髓保护的小鼠，但不是在小鼠给予850拉德全身照射。有一个显着的增加转移EAE的严重程度在轻度照射收件人，可能是由于选择性的放射敏感性抑制T [胸腺衍生]细胞。转移性EAE受者中表现出细胞介导的免疫，但没有针对髓鞘碱性蛋白的循环抗体。负责EAE转移的免疫淋巴细胞为T淋巴细胞。因此，在致敏细胞通过抗免疫球蛋白柱后，转移成功，并且在用抗Thy-1血清和补体处理后被废除。新生胸腺切除小鼠未能发展EAE，细胞介导的免疫或抗髓鞘碱性蛋白（BPM）的体液抗体。EAE和免疫应答的抑制仅仅是由于胸腺淋巴细胞来源的去除，因为用T淋巴细胞重建的胸腺切除小鼠完全恢复了这些功能。T淋巴细胞对于EAE的产生和过继转移、对于BPM的细胞介导的免疫的发展以及对于BPM的抗体的产生是必需的。
Cellular transfer of experimental autoimmune encephalomyelitis (EAE) was effected in mice with lymph node and spleen cells from appropriately immunized donors. Immune serum did not transfer this autoimmune disease, nor did serum have any facilitating or inhibitory effect on the capacity of lymphoid cells to transfer EAE. Transfer of EAE was effected in normal mice, lightly irradiated (350 rad) and lethally irradiated (850 rad) and bone marrow-protected mice, but not in mice given 850 rad to total-body irradiation. There was a striking augmentation of severity of transferred EAE in the lightly irradiated recipients, possibly attributable to selective radiosensitivity of suppressor T [thymus-derived] cells. Cell-mediated immunity but not circulating antibody to basic protein of myelin was demonstrated in recipients with transferred EAE. The immune lymphoid cells responsible for transfer of EAE were T lymphocytes. Thus, transfer was successful after passage of sensitized cells through anti-immunoglobulin columns, and was abrogated following treatment with anti-Thy-1 serum and complement. Neonatally thymectomized mice failed to develop EAE, cell-mediated immunity or humoral antibody against myelin basic protein (BPM). Inhibition of EAE and immune responsiveness was solely due to the removal of the source of thymus lymphocytes, because reconstitution of neonatally thymectomized mice with T lymphocytes completely restored these functions. T lymphocytes are required for the production and adoptive transfer of EAE, for the development of cell-mediated immunity to BPM, and for the production of antibody to BPM.