The insulin sensitizing effect of homoisoflavone-enriched fraction in Liriope platyphylla Wang et Tang via PI3-kinase pathway
The insulin sensitizing effect of homoisoflavone-enriched fraction in Liriope platyphylla Wang et Tang via PI3-kinase pathway
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DOI：
10.1016/j.lfs.2004.04.039
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发表时间：
2004-10-15
期刊：
影响因子：
6.1
通讯作者：
Park, S
中科院分区：
文献类型：
--
作者：
Choi, SB;Wha, JD;Park, S
在本研究中，我们使用葡萄糖摄取作为指标，从百合科的百合麦冬提取物中筛选出增强胰岛素作用的候选药物，在 3T3-L1 脂肪细胞中。还研究了该级分中胰岛素增敏作用的机制。 70% MeOH 的 LPWT 提取物依次用 Diaion HP-20 和硅胶柱色谱分离。来自硅胶柱色谱的 9:1 级分将 1 ng/mL 的葡萄糖摄取量增加至 50 ng/mL 胰岛素的葡萄糖摄取量。 9:1 的级分被确定为富含高异黄酮的级分，可作为胰岛素增敏剂。它通过增加质膜中葡萄糖转运蛋白 4 (GLUT4) 的含量，增加胰岛素刺激的 3T3-L1 脂肪细胞（胰岛素反应性细胞）的葡萄糖摄取。 GLUT4 易位通过胰岛素受体底物 1 (IRS1)-PI3 激酶-Akt 信号传导机制增加。因此，LPWT 提取物中富含同异黄酮的部分作为脂肪细胞中的胰岛素增敏剂发挥着重要作用。 (C) 2004 Elsevier Inc. 保留所有权利。
In the present study, we screened candidates for enhancing insulin action, using glucose uptake as an indicator, from Liriope platyphylla Wang et Tang (LPWT) extract, Liliaceae, in 3T3-L1 adipocytes. The mechanism of insulin sensitizing action in the fractions was also investigated. LPWT extract with 70% MeOH was sequentially separated with Diaion HP-20 and silica gel column chromatography. The 9:1 fraction from silica gel column chromatography increased glucose uptake with 1 ng/mL up to glucose uptake with 50 ng/mL insulin. The 9:1 fraction, determined as homoisoflavone-enriched fraction, worked as an insulin sensitizer. It increased insulin stimulated glucose uptake in 3T3-L1 adipocytes, insulin responsive cells, through increased glucose transporter 4 (GLUT4) contents in the plasma membrane. GLUT4 translocation was increased through insulin receptor substrate 1 (IRS1)-PI3 kinase-Akt signaling mechanism. Thus, homoisoflavone-enriched fraction in LPWT extract played an important role as an insulin sensitizer in adipocytes. (C) 2004 Elsevier Inc. All rights reserved.