TENTATIVE DIRECT MICROSCOPIC METHOD FOR COUNTING LIVING MARINE-BACTERIA
TENTATIVE DIRECT MICROSCOPIC METHOD FOR COUNTING LIVING MARINE-BACTERIA
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DOI：
10.1139/m79-063
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发表时间：
1979-01-01
影响因子：
2.8
通讯作者：
TAGA, N
中科院分区：
文献类型：
--
作者：
KOGURE, K;SIMIDU, U;TAGA, N
向海水样品中加入酵母提取物（0.025%）和萘啶酸（0.002%），并将样品在20 ℃下温育6小时。C在黑暗中在这些条件下，细菌细胞不分裂，而是生长形成细长的细胞，这些细胞很容易通过直接显微镜方法和落射荧光显微镜技术识别。由此获得的细胞数被提议作为活细菌细胞（DVC）的直接计数。与开放的海洋样品，DVC高于可行的平板计数高达3个数量级，低于直接计数约1个数量级。
Yeast extract (0.025%) and nalidixic acid (0.002%) were added to seawater samples and the samples were incubated for 6 h at 20.degree. C in the dark. Under these conditions, bacterial cells did not divide but grew to form elongated cells that are easily recognized by a direct microscopic method and epifluorescent microscopic technique. The number of cells thus obtained is proposed as a direct count of viable bacterial cells (DVC). With open ocean samples, DVC was higher than viable plate counts by up to 3 orders of magnitude and lower than the direct counts by about 1 order.