The recent association of a DNA betasatellite with Tomato yellow leaf curl virus in Israel - A new threat to tomato production
The recent association of a DNA betasatellite with Tomato yellow leaf curl virus in Israel - A new threat to tomato production
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DOI：
10.1016/j.cropro.2019.104995
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发表时间：
2020-02-01
期刊：
影响因子：
2.8
通讯作者：
Lapidot, Moshe
中科院分区：
文献类型：
--
作者：
Gelbart, Dana;Chen, Lea;Lapidot, Moshe
在2016年11月期间，番茄黄化曲叶病毒（TYLCV）抗性F-1杂种无限番茄植物cv.在以色列Revaya的商业温室中生长的Diagrama表现出番茄黄化曲叶病（TYLCD）的严重症状。从有症状的番茄植株中收集样品，并使用TYLCV特异性PCR引物，证实这些植株确实感染了TYLCV。使用通用β卫星引物对B 01/B 02，随后进行克隆和测序，发现植物也被β卫星感染。该卫星序列保藏于NCBI（GeneBank Acc.No.MK456609）。系统发育分析表明，与棉花卷叶盖济拉betasatellite（CLCuGB）最接近的相似性为96%。在以色列主要种植区对有症状的番茄植株进行取样，发现CLCuGB在全国各地传播。据我们所知，这是第一次在以色列番茄植株中发现与TYLCV相关的β卫星。
During November 2016, tomato yellow leaf curl virus (TYLCV)-resistant F-1 hybrid indeterminate tomato plants cv. Diagrama, grown in a commercial greenhouse in Revaya, Israel, expressed severe symptoms of tomato yellow leaf curl disease (TYLCD). Samples were collected from symptomatic tomato plants, and using TYLCV-specific PCR primers, it was confirmed that these plants were indeed infected with TYLCV. Using the universal betasatellite primer pair B01/B02, followed by cloning and sequencing, it was found that the plants were also infected with a betasatellite. The satellite sequence was deposited in NCBI (GeneBank Acc. No. MK456609). Phylogenetic analysis revealed that the closest similarity, with 96% nucleotide identity, was to cotton leaf curl Gezira betasatellite (CLCuGB). Sampling symptomatic tomato plants in major growing areas in Israel revealed that CLCuGB is disseminated throughout the country. To the best of our knowledge, this is the first identification of a betasatellite, associated with TYLCV, in tomato plants in Israel.