New insight into progenitor/stem cells in dental pulp using Col1a1-GFP transgenes
New insight into progenitor/stem cells in dental pulp using Col1a1-GFP transgenes
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DOI：
10.1159/000075033
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发表时间：
2004-01-01
影响因子：
2.7
通讯作者：
Braut, A
中科院分区：
文献类型：
--
作者：
Mina, M;Braut, A
近年来，在鉴定成体组织干细胞及其在组织工程中的潜在应用方面取得了越来越大的进展。这些进步为牙齿替换/再生提供了充满希望的未来。这种方法的关键是识别牙齿各个组成部分的供体干细胞。我们的研究表明，pOBCol3.6GFPtpz 和 pOBCol2.3GFPemd 转基因动物提供了一个独特的模型来深入了解牙髓中的干细胞。我们对这些转基因品系发育中的牙齿的体内研究表明，Col1a1-GFP 转基因在功能性和完全分化的成牙本质细胞中表达。成牙本质细胞分化过程中 Col1a1-GFP 转基因的表达模式与 DSPP 的表达相关。在发育中的颅面骨中，Col1a1-GFP 转基因也在牙槽骨和颅骨的成骨细胞和骨细胞中表达。在牙槽骨中，骨细胞中 Col1a1-GFP 的表达与 Dmp1 的表达相关。 Col1a1-3.6-GFP 在整个骨膜层和含有骨祖细胞的缝线间充质中表达。另一方面，Col1a1-2.3-GFP表达仅限于骨膜的成骨细胞层，在骨膜的成纤维细胞层或缝合间充质中未检测到。这些观察结果表明，Col1a1-3.6-GFP 和 Col1a1-2.3-GFP 转基因在膜内骨化过程中识别不同的细胞亚群。通过使用从出生后转基因小鼠中分离的牙髓的冠状部分，我们的观察还提供了直接证据，证明牙髓含有能够产生新一代成牙本质细胞样细胞以及成骨细胞样细胞的祖细胞/干细胞。版权所有 (C) 2004 S. Karger AG，巴塞尔。
In recent years there has been increasing progress in identifying stem cells from adult tissues and their potential application in tissue engineering. These advances provide a promising future for tooth replacement/regeneration. Essential for this approach is the identification of donor stem cells for various components of the teeth. Our studies show that pOBCol3.6GFPtpz and pOBCol2.3GFPemd transgenic animals provide a unique model to gain insight into stem cells in the dental pulp. Our in vivo studies of the developing teeth of these transgenic lines show both Col1a1-GFP transgenes are expressed in functional and fully differentiated odontoblasts. The patterns of expression of Col1a1-GFP transgenes during odontoblast differentiation correlates with the expression of DSPP. In the developing craniofacial bones both Col1a1-GFP transgenes are also expressed in osteoblasts and osteocytes of alveolar and calvarial bones. In the alveolar bones, the expression of Col1a1-GFP in osteocytes correlates with the expression of Dmp1. Col1a1-3.6-GFP is expressed in the entire layer of the periosteum and in suture mesenchyme containing osteoprogenitor cells. On the other hand, Col1a1-2.3-GFP expression was limited to the osteoblastic layer of the periosteum and was not detected in the fibroblastic layer of the periosteum or in the suture mesenchyme. These observations indicate that Col1a1-3.6-GFP and Col1a1-2.3-GFP transgenes identify different subpopulations of cells during intramembranous ossification. By using the coronal portion of dental pulps isolated from postnatal transgenic mice our observations also provide direct evidence that the dental pulp contains progenitor/stem cells capable of giving rise to a new generation of odontoblast-like cells, as well as osteoblast-like cells. Copyright (C) 2004 S. Karger AG, Basel.