A Microneedle Technology for Sampling and Sensing Bacteria in the Food Supply Chain
A Microneedle Technology for Sampling and Sensing Bacteria in the Food Supply Chain
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DOI：
10.1002/adfm.202005370
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发表时间：
2020-09-09
影响因子：
19
通讯作者：
Marelli, Benedetto
中科院分区：
文献类型：
--
作者：
Kim, Doyoon;Cao, Yunteng;Marelli, Benedetto
食品质量监测，特别是在整个食品供应链中检测细菌病原体和腐败，对于确保全球食品安全和最大限度地减少食品损失至关重要。将传感器封装到包装中是有希望的，但在使用食品安全传感器材料的同时实现所需的采样体积是具有挑战性的。在这里，通过利用丝素蛋白的水基加工，使用多孔丝微针阵列实现了用于检测食品中致病菌的平台;微针阵列通过毛细管作用从食品内部采样流体，将流体呈现给印刷在阵列背面上的基于聚二乙炔的生物墨水。通过生物墨水图案的比色响应，在16 h内的针头注射鱼片大肠杆菌污染的确定。这种响应与通过样品pH值增加测量的腐败不同。还表明，微针可以刺穿商业食品包装，随后对流体进行采样并将其呈现给传感器，从而能够适应食品供应链下游的技术，例如在商店或家中。这项研究强调，再生的结构生物聚合物可以作为食品接触和传感的安全材料，具有强大的机械性能和定制的化学性质。
Food quality monitoring, particularly, the detection of bacterial pathogens and spoilage throughout the food supply chain, is critical to ensure global food safety and minimize food loss. Incorporating sensors into packaging is promising, but it is challenging to achieve the required sampling volume while using food-safe sensor materials. Here, by leveraging water-based processing of silk fibroin, a platform for the detection of pathogenic bacteria in food is realized using a porous silk microneedle array; the microneedle array samples fluid from the interior of the food by capillary action, presenting the fluid to polydiacetylene-based bioinks printed on the backside of the array. Through the colorimetric response of bioink patterns,Escherichia colicontamination in fish fillets is identified within 16 h of needle injection. This response is distinct from spoilage measured via the increase in sample pH. It is also shown that the microneedles can pierce commercial food packaging, and subsequently sample fluid and present it to the sensor, enabling the adaptation of the technology downstream in food supply chains such as in stores or at home. This study highlights that regenerated structural biopolymers can serve as safe materials for food contact and sensing with robust mechanical properties and tailored chemistry.