Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans
Parity among the randomly amplified polymorphic DNA method, multilocus enzyme electrophoresis, and southern blot hybridization with the moderately repetitive DNA probe Ca3 for fingerprinting Candida albicans
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DOI：
10.1128/jcm.35.9.2348-2358.1997
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发表时间：
1997-09-01
影响因子：
9.4
通讯作者：
Soll, DR
中科院分区：
文献类型：
--
作者：
Pujol, C;Joly, S;Soll, DR
随机扩增多态性DNA （RAPD）分析、多位点酶电泳（MLEE）和适度重复DNA探针的Southern blot杂交已成为传染性真菌白色念珠菌的有效指纹识别方法。我们比较了这三种方法在鉴定相同或高度相关的分离株、对弱相关分离株进行干扰、区分不相关分离株和评估菌株内微进化方面的能力，并计算了来自美国大陆三个城市的29株分离株之间的相似系数，结果表明RAPD分析、MLEE和使用中度重复探针Ca3的Southern blot杂交结果具有很强的一致性。结果表明，这三种指纹识别方法与适度重复探针CARE2的Southern blot杂交结果一致性较弱。所有方法也被证明能够解决微演化在一个应变，与Ca3探针显示出最大的分辨能力。四种独立指纹分析方法评估的多态性标记与RAPD分析和基因座之间的非随机关联显示了强相关性。MLEE提供了白色念珠菌强烈的连锁不平衡和克隆群体结构的证据。此外，在RAPD分析、MLEE和Ca3指纹识别的三个聚类中，其中一个聚类的所有成员中都存在一个突触型等位基因Pep-3A，这支持了三种方法聚类能力的一致性、聚类的稳健性和聚类的克隆性。
Randomly amplified polymorphic DNA (RAPD) analysis, mutilocus enzyme electrophoresis (MLEE), and Southern blot hybridization with moderately repetitive DNA probes have emerged as effective fingerprinting methods for the infectious fungus Candida albicans. The three methods Rave been compared for their capacities to identify identical or highly related isolates, to fluster weakly related isolates, to discriminate between unrelated isolates, and to assess microevolution within a strain, Bg computing similarity coefficients between 29 isolates from three cities within the continental United States, strong concordance of the results is demonstrated Fur RAPD analysis, MLEE, and Southern blot hybridization with the moderately repetitive probe Ca3, and weaker concordance of the results is demonstrated for these three fingerprinting methods and Southern blot hybridization with the moderately repetitive probe CARE2. All methods were also demonstrated to be able to resolve microevolution within a strain, with the Ca3 probe exhibiting the greatest resolving power. The strong correlations demonstrated between polymorphic markers assessed by the four independent fingerprinting methods and the nonrandom association between loci demonstrated by RAPD analysis and. MLEE provide evidence for strong linkage disequilibrium and a clonal population structure for C. albicans. In addition, a synapomorphic allele, Pep-3A, was found to be present in all members of one of the three clusters discriminated by RAPD analysis, MLEE, and Ca3 fingerprinting, supporting the concordance of the clustering capacities of the three methods, the robustness Of the clusters, and the clonal nature of the clusters.