NF-YC12 is a key multi-functional regulator of accumulation of seed storage substances in rice
NF-YC12 is a key multi-functional regulator of accumulation of seed storage substances in rice
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DOI：
10.1093/jxb/erz168
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发表时间：
2019-04
影响因子：
6.9
通讯作者：
Yufei Xiong;Ye Ren;Wang Li;Fengsheng Wu;Wenjie Yang;Xiaolong Huang;Jialing Yao
中科院分区：
文献类型：
--
作者：
Yufei Xiong;Ye Ren;Wang Li;Fengsheng Wu;Wenjie Yang;Xiaolong Huang;Jialing Yao
淀粉和贮藏蛋白是谷类胚乳的主要贮藏物质，是人类主要的食物来源。然而，储存物质合成和积累的转录调控网络在很大程度上仍然未知。在这里，我们鉴定了一个水稻胚乳特异性基因NF-YC12，该基因编码一个假定的核因子- y转录因子亚基c。NF-YC12在籽粒发育过程中在淀粉质胚乳和淀粉质胚乳中表达。敲除NF-YC12显著降低籽粒重，改变淀粉和蛋白质积累及淀粉颗粒形成。rna测序分析显示，在nf-yc12突变体中，与淀粉生物合成和能量储备代谢相关的基因在下调类别中富集。此外，nf - yc12过表达系与野生型的种子中淀粉和蛋白质含量也存在差异。发现NF-YC12与NF-YB1相互作用。ChIP-qPCR和酵母单杂交分析表明，NF-YC12与NF-YB1在淀粉粉层协同调控水稻蔗糖转运蛋白OsSUT1。此外，NF-YC12直接结合到FLO6 （FLOURY ENDOSPERM6）和OsGS1的启动子上；3（谷氨酰胺合成酶1）发育胚乳。本研究揭示了一个涉及NF-YC12的转录调控网络，该网络协调多种途径调节水稻种子胚乳发育和储存物质的积累。
Abstract Starch and storage proteins, the primary storage substances of cereal endosperm, are a major source of food for humans. However, the transcriptional regulatory networks of the synthesis and accumulation of storage substances remain largely unknown. Here, we identified a rice endosperm-specific gene, NF-YC12, that encodes a putative nuclear factor-Y transcription factor subunit C. NF-YC12 is expressed in the aleurone layer and starchy endosperm during grain development. Knockout of NF-YC12 significantly decreased grain weight as well as altering starch and protein accumulation and starch granule formation. RNA-sequencing analysis revealed that in the nf-yc12 mutant genes related to starch biosynthesis and the metabolism of energy reserves were enriched in the down-regulated category. In addition, starch and protein contents in seeds differed between NF-YC12-overexpression lines and the wild-type. NF-YC12 was found to interact with NF-YB1. ChIP-qPCR and yeast one-hybrid assays showed that NF-YC12 regulated the rice sucrose transporter OsSUT1 in coordination with NF-YB1 in the aleurone layer. In addition, NF-YC12 was directly bound to the promoters of FLO6 (FLOURY ENDOSPERM6) and OsGS1;3 (glutamine synthetase1) in developing endosperm. This study demonstrates a transcriptional regulatory network involving NF-YC12, which coordinates multiple pathways to regulate endosperm development and the accumulation of storage substances in rice seeds.