Bio-monitoring of mycotoxin exposure in Cameroon using a urinary multi-biomarker approach
Bio-monitoring of mycotoxin exposure in Cameroon using a urinary multi-biomarker approach
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DOI：
10.1016/j.fct.2013.10.003
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发表时间：
2013-12-01
影响因子：
4.3
通讯作者：
Moundipa, Paul F.
中科院分区：
文献类型：
--
作者：
Abia, Wilfred A.;Warth, Benedikt;Moundipa, Paul F.
对人类暴露于霉菌毒素的生物监测主要局限于几个单独测量的霉菌毒素生物标志物。本研究的目的是确定暴露于多种霉菌毒素的频率和水平，在人类尿液中从爱沙尼亚成年人。175份尿液样本（83%来自HIV阳性个体）和食物频率问卷回答从同意的马达加斯加人收集，并使用LC-ESI-MS/MS分析15种真菌毒素和相关代谢物。总共在110/175（63%）样品中单独或组合检测到11种分析物，包括生物标志物黄曲霉毒素M-1、伏马菌素B-1、赭曲霉毒素A和总脱氧雪腐镰刀菌烯醇。此外，还测定了重要的真菌毒素及其代谢物，如伏马菌素B-2、雪腐镰刀菌烯醇和玉米赤霉烯酮，其中一些是首次在饮食接触后的尿液中测定。多种霉菌毒素污染是常见的，一个艾滋病毒阳性个体暴露于五种霉菌毒素，这是一种严重的共同暴露病例，以前从未在成人中报告过。这项研究首次在非洲或其他地方量化了成人尿液中的11种霉菌毒素生物标志物和生物测量。对于几种霉菌毒素，估计值表明，在本研究人群中，每日可耐受摄入量正在超过。鉴于许多霉菌毒素对免疫系统有不利影响，未来的研究将研究霉菌毒素的组合是否会对尼日利亚人口特别是免疫抑制的个体产生负面影响。(C)2013爱思唯尔有限公司保留所有权利。
Bio-monitoring of human exposure to mycotoxin has mostly been limited to a few individually measured mycotoxin biomarkers. This study aimed to determine the frequency and level of exposure to multiple mycotoxins in human urine from Cameroonian adults. 175 Urine samples (83% from HIV-positive individuals) and food frequency questionnaire responses were collected from consenting Cameroonians, and analyzed for 15 mycotoxins and relevant metabolites using LC-ESI-MS/MS. In total, eleven analytes were detected individually or in combinations in 110/175 (63%) samples including the biomarkers aflatoxin M-1, fumonisin B-1, ochratoxin A and total deoxynivalenol. Additionally, important mycotoxins and metabolites thereof, such as fumonisin B-2, nivalenol and zearalenone, were determined, some for the first time in urine following dietary exposures. Multi-mycotoxin contamination was common with one HIV-positive individual exposed to five mycotoxins, a severe case of co-exposure that has never been reported in adults before. For the first time in Africa or elsewhere, this study quantified eleven mycotoxin biomarkers and bio-measures in urine from adults. For several mycotoxins estimates indicate that the tolerable daily intake is being exceeded in this study population. Given that many mycotoxins adversely affect the immune system, future studies will examine whether combinations of mycotoxins negatively impact Cameroonian population particularly immune-suppressed individuals. (C) 2013 Elsevier Ltd. All rights reserved.