OVARIAN DEVELOPMENT AND VITELLOGENESIS IN THE SAWFLY, ATHALIA-ROSAE-RUFICORNIS JAKOVLEV (HYMENOPTERA, TENTHREDINIDAE)
OVARIAN DEVELOPMENT AND VITELLOGENESIS IN THE SAWFLY, ATHALIA-ROSAE-RUFICORNIS JAKOVLEV (HYMENOPTERA, TENTHREDINIDAE)
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DOI：
10.1080/07924259.1990.9672116
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发表时间：
1990-06-01
影响因子：
0.8
通讯作者：
OISHI, K
中科院分区：
文献类型：
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作者：
HATAKEYAMA, M;SAWA, M;OISHI, K
描述了蔷薇花蜂科（膜翅目：蜂科）的卵巢发育。每个卵室的护理细胞数量通常在60左右（根据2n-1规则接近63），但在许多情况下，它明显偏离这个数字。通过SDS-PAGE鉴定了两种主要的蛋黄蛋白[卵黄蛋白：大蛋白（表观分子量160-170 kD）和小蛋白（48-50 kD）]。Western blotting和免疫化学检测显示，成年雌性（单倍体和二倍体）的血淋巴中含有与这些卵黄蛋白对应的卵黄蛋白原，而雄性（单倍体和二倍体）的血淋巴中没有。卵黄原蛋白可以在晚期蛹的血淋巴中检测到，而卵黄原蛋白可以在一天后在成虫的卵母细胞中检测到。将未成熟的卵巢移植到成年雄性腹部，不仅在卵母细胞中积累了大量卵黄蛋白，而且在宿主雄性体内出现了少量的血淋巴卵黄蛋白。注射未成熟卵巢匀浆也可引起宿主雄性出现少量血淋巴卵黄蛋白原。
Ovarian development in Athalia rosae ruficornis Jakovlev (Hymenoptera: Tenthredinidae) is described. Number of nurse cells per egg chamber is most often around 60 (close to 63 according to the 2n-1 rule), but in many cases it deviates from this number significantly. Two major yolk proteins [vitellins: large (apparent molecular weight 160-170 kD) and small (48-50 kD] were identified by SDS-PAGE. Western blotting and immunochemical detection using polyclonal antibodies prepared against each of the vitellins revealed that adult female but not male (both haploid and diploid) hemolymph contains vitellogenins corresponding to these vitellins. Vitellogenins become detectable in the hemolymph of late pupae, ad vitellins one day later in the oocytes of adults. Transplantation of immature ovaries into the adult male abdomen caused not only significant accumulation of vitellins in the oocytes but also appearance of small amounts of hemolymph vitellogenins in host males. Injection of homogenate of immature ovaries also caused appearance of small amounts of hemolymph vitellogenins in host males.