Following single antibody binding to purple membranes in real time
Following single antibody binding to purple membranes in real time
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DOI：
10.1038/sj.embor.7400149
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发表时间：
2004-06-01
期刊：
影响因子：
7.7
通讯作者：
Hinterdorfer, P
中科院分区：
文献类型：
--
作者：
Kienberger, F;Mueller, H;Hinterdorfer, P
抗体与膜中的表面抗原结合是脊椎动物特异性免疫防御的主要事件。在这里，我们使用力显微镜研究了含有基因附加的抗仙台识别表位的盐杆菌突变体紫色膜的抗体识别动态。随着时间的推移，观察到各个抗仙台抗体与其抗原表位的连接。它们在选定的小区域内数量的增加揭示了明显的动力学作用率。膜结合抗体显示出许多不同的构象，从球形到 V 形和 Y 形。观察到同一抗体的两个Fab片段的最大距离接近18 nm，表明该抗体铰链区总体上具有很强的内在灵活性。结合的抗仙台抗体的 Fab 片段被分配到紫色膜的抗原位点，从而允许识别和定位紫色膜表面上的个体识别表位。
Antibody binding to surface antigens in membranes is the primary event in the specific immune defence of vertebrates. Here we used force microscopy to study the dynamics of antibody recognition of mutant purple membranes from Halobacterium salinarum containing a genetically appended anti-Sendai recognition epitope. Ligation of individual anti-Sendai antibodies to their antigenic epitopes was observed over time. Their increase in number within a small selected area revealed an apparent kinetic on-rate. The membrane-bound antibodies showed many different conformations that ranged from globular to V- and Y-like shapes. The maximum distance of two Fab fragments of the same antibody was observed to be similar to18 nm, indicating an overall strong intrinsic flexibility of the antibody hinge region. Fab fragments of bound anti-Sendai antibodies were allocated to antigenic sites of the purple membrane, allowing the identification and localization of individual recognition epitopes on the surface of purple membranes.